Demonstration of immunoglobulin G with affinity for dopamine in cerebrospinal fluid from psychotic patients.

Bergquist, JonasDepartment of Clinical Neuroscience, Section of Psychiatry and Neurochemistry, University of Göteborg (author)

Using an enzyme-linked immunosorbent assay, significantly raised concentrations of immunoglobulin G with affinity for the neurotransmitter dopamine were demonstrated in cerebrospinal fluid from psychotic patients. We have varied the antigen presentation in order to find a conjugate with low unspecific binding. The conjugation of dopamine to carbodiimide-activated poly-L-glutamic acid and that to activated succinimide ester of biotin are described. The use of glutaraldehyde conjugation is not recommended because of the risk of formation of tetrahydroisoquinolines. A strong correlation (r = 0.94, P < 0.001) between the results obtained with dopamine conjugated to poly-L-glutamic acid and dopamine conjugated to biotin was observed. Forty-two human cerebrospinal fluid samples from 20 psychotic patients, (12 with a bipolar disorder and 8 with schizophrenia) and 22 control patients, with various neurological diseases but no apparent psychiatric diseases were investigated. A significantly higher incidence (P < 0.001) of antibodies with affinity for dopamine were found in the group of psychotic patients compared with the neurological control group.

Bergquist, SDepartment of Psychiatry and Neurochemistry, University of Lund (author)
Axelsson, RDepartment of Psychiatry, Sahlgren Hospital, University of Göteborg (author)
Ekman, RDepartment of Clinical Neuroscience, Section of Psychiatry and Neurochemistry, University of Göteborg (author)
Department of Clinical Neuroscience, Section of Psychiatry and Neurochemistry, University of GöteborgDepartment of Psychiatry and Neurochemistry, University of Lund (creator_code:org_t)

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