Protein identification by liquid chromatography-mass spectrometry using retention time prediction

Liquid chromatography has been coupled with mass spectrometry to improve the dynamic range and to reduce the complexity of sample introduced to the mass spectrometer at any given time. The chromatographic separation also provides information on the analytes, such as peptides in enzymatic digests of proteins; information that can be used when identifying the proteins by peptide mass fingerprinting. This paper discusses a recently introduced method based on retention time prediction to extract information from chromatographic separations and the applications of this method to protein identification in organisms with small and large genomes.

Ramström, MargaretaUppsala universitet,Jonfysik,Analytisk kemi(Swepub:uu)maram031 (författare)
Bailey, Christopher GChemistry and Materials Science, Lawrence Livermore National Laboratory, USA (författare)
McCutchen-Maloney, Sandra LBiology and Biotechnology Research Program, Lawrence Livermore National Laboratory, USA (författare)
Bergquist, JonasUppsala universitet,Analytisk kemi(Swepub:uu)jbe25356 (författare)
Zeller, Loreen CChemistry and Materials Science, Lawrence Livermore National Laboratory, Livermore, CA 94551-0808, USA (författare)
Uppsala universitetJonfysik (creator_code:org_t)

Ingår i:Journal of chromatography. B: Elsevier BV803:1, s. 131-1351570-02321873-376X

Av författaren/redakt...: Palmblad, Magnus; Ramström, Margar ...; Bailey, Christop ...; McCutchen-Malone ...; Bergquist, Jonas; Zeller, Loreen C

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