Electron transfer between cellobiose dehydrogenase and graphite electrodes

Sökning: L773:0003 2670 > Electron transfer b...

Electron transfer between the enzyme cellobiose dehydrogenase (CDH) and a flavin adenine dinucleotide (FAD) containing a catalytic active fragment of CDH (FAD-fragment) and a graphite electrode, respectively, was established. The current response in the presence of the enzyme substrate for graphite electrodes with CDH or the FAD-fragment adsorbed on the freshly polished graphite surface were compared with that of electrodes where CDH or the FAD-fragment were crosslinked in a redox polymer at the electrode surface. The initial slope, dj/d[S](S=0), where j is the current density and [S](S=0) the zero substrate concentration, was taken as a measure of the substrate response. For the electrodes with enzymes adsorbed directly on the surface, dj/d[S](S=0), was a factor of 3 lower than for electrodes prepared with the polymer mixture. The redox polymer based electrodes, with CDH and with FAD-fragment, both showed a high and close to equal substrate response. In contrast the surface adsorbed CDH gave a much higher substrate response than the FAD-fragment.

Elmgren, Maja,1964-Uppsala universitet,Fysikalisk-kemiska institutionen,Fysikalisk kemi(Swepub:uu)majaelmg (författare)
Lindquist, Sten-EricUppsala universitet,Fysikalisk-kemiska institutionen(Swepub:uu)stenlind (författare)
Tessema, Merid (författare)
Gorton, Lo (författare)
Henriksson, GunnarUppsala universitet,Biokemi (författare)
Uppsala universitetFysikalisk-kemiska institutionen (creator_code:org_t)

Av författaren/redakt...: Larsson, Ted; Elmgren, Maja, 1 ...; Lindquist, Sten- ...; Tessema, Merid; Gorton, Lo; Henriksson, Gunn ...

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