Bacterial ribosome requires multiple L12 dimers for efficient initiation and elongation of protein synthesis involving IF2 and EF-G

Mandava, Chandra Sekhar,1978-Uppsala universitet,Struktur- och molekylärbiologi,Dr. Suparna Sanyal (author)

The ribosomal stalk in bacteria is composed of four or six copies of L12 proteins arranged in dimers that bind to the adjacent sites on protein L10, spanning 10 amino acids each from the L10 C-terminus. To study why multiple L12 dimers are required on the ribosome, we created a chromosomally engineered Escherichia coli strain, JE105, in which the peripheral L12 dimer binding site was deleted. Thus JE105 harbors ribosomes with only a single L12 dimer. Compared to MG1655, the parental strain with two L12 dimers, JE105 showed significant growth defect suggesting suboptimal function of the ribosomes with one L12 dimer. When tested in a cell-free reconstituted transcription-translation assay the synthesis of a full-length protein, firefly luciferase, was notably slower with JE105 70S ribosomes and 50S subunits. Further, in vitro analysis by fast kinetics revealed that single L12 dimer ribosomes from JE105 are defective in two major steps of translation, namely initiation and elongation involving translational GTPases IF2 and EF-G. Varying number of L12 dimers on the ribosome can be a mechanism in bacteria for modulating the rate of translation in response to growth condition.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
ribosome
translation initiation
L12
IF2
subunit association
protein synthesis
Molecular biology
Molekylärbiologi
Biology with specialization in Molecular Biology
Biologi med inriktning mot molekylärbiologi

Peisker, KristinUppsala universitet,Struktur- och molekylärbiologi,Dr. Suparna Sanyal(Swepub:uu)kripe228 (author)
Ederth, JosefineUppsala universitet,Struktur- och molekylärbiologi (author)
Kumar, RanjeetUppsala universitet,Struktur- och molekylärbiologi,Dr.Suparna Sanyal(Swepub:uu)ranku252 (author)
Ge, XueliangUppsala universitet,Struktur- och molekylärbiologi,Dr.Suparna Sanyal(Swepub:uu)xuege734 (author)
Szaflarski, WitoldKnud H. Nierhaus (author)
Sanyal, SuparnaUppsala universitet,Struktur- och molekylärbiologi(Swepub:uu)sucsa102 (author)
Uppsala universitetStruktur- och molekylärbiologi (creator_code:org_t)

In:Nucleic Acids Research: Oxford University Press (OUP)40:5, s. 2054-20640305-10481362-4962

By the author/editor: Mandava, Chandra ...; Peisker, Kristin; Ederth, Josefine; Kumar, Ranjeet; Ge, Xueliang; Szaflarski, Wito ...; show more...; Sanyal, Suparna; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

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