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ProteinSeq :
ProteinSeq : high-performance proteomic analyses by proximity ligation and next generation sequencing
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- Darmanis, Spyros (author)
- Uppsala universitet,Molekylära verktyg,Science for Life Laboratory, SciLifeLab,Ulf Landegren
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- Nong, Rachel Yuan, 1982- (author)
- Uppsala universitet,Molekylära verktyg,Science for Life Laboratory, SciLifeLab,Ulf Landegren
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- Vänelid, Johan (author)
- Uppsala universitet,Molekylära verktyg,Science for Life Laboratory, SciLifeLab,Ulf Landegren
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- Siegbahn, Agneta (author)
- Uppsala universitet,Klinisk kemi
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- Ericsson, Olle (author)
- Halo Genomics AB, Dag Hammarskjölds väg 36B, SE-752 37 Uppsala Sweden
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- Fredriksson, Simon (author)
- Olink Biosciences, Dag Hammarskjölds väg 52B, SE-752 37 Uppsala, Sweden
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- Bäcklin, Christofer (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper
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- Gut, Marta (author)
- Centro Nacional de Análisis Genómico, C/Baldiri Reixac 4, 08028 Barcelona, Spain
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- Heath, Simon (author)
- Centro Nacional de Análisis Genómico, C/Baldiri Reixac 4, 08028 Barcelona, Spain
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- Gut, Ivo Glynne (author)
- Centro Nacional de Análisis Genómico, C/Baldiri Reixac 4, 08028 Barcelona, Spain
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- Wallentin, Lars (author)
- Uppsala universitet,Uppsala kliniska forskningscentrum (UCR)
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- Gustafsson, Mats G. (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper
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- Kamali-Moghaddam, Masood (author)
- Uppsala universitet,Molekylära verktyg,Science for Life Laboratory, SciLifeLab
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- Landegren, Ulf (author)
- Uppsala universitet,Molekylära verktyg,Science for Life Laboratory, SciLifeLab
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(creator_code:org_t)
- 2011-09-29
- 2011
- English.
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In: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 6:9, s. e25583-
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Despite intense interest, methods that provide enhanced sensitivity and specificity in parallel measurements of candidate protein biomarkers in numerous samples have been lacking. We present herein a multiplex proximity ligation assay with readout via realtime PCR or DNA sequencing (ProteinSeq). We demonstrate improved sensitivity over conventional sandwich assays for simultaneous analysis of sets of 35 proteins in 5 μl of blood plasma. Importantly, we observe a minimal tendency to increased background with multiplexing, compared to a sandwich assay, suggesting that higher levels of multiplexing are possible. We used ProteinSeq to analyze proteins in plasma samples from cardiovascular disease (CVD) patient cohorts and matched controls. Three proteins, namely P-selectin, Cystatin-B and Kallikrein-6, were identified as putative diagnostic biomarkers for CVD. The latter two have not been previously reported in the literature and their potential roles must be validated in larger patient cohorts. We conclude that ProteinSeq is promising for screening large numbers of proteins and samples while the technology can provide a much-needed platform for validation of diagnostic markers in biobank samples and in clinical use.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
Keyword
- proximity ligation assay
- next generation sequencing
- cardiovascular disease
- biomarker
- Molekylär medicin
- Molecular Medicine
Publication and Content Type
- ref (subject category)
- art (subject category)
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Darmanis, Spyros
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Nong, Rachel Yua ...
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Vänelid, Johan
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Siegbahn, Agneta
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Ericsson, Olle
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Fredriksson, Sim ...
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Bäcklin, Christo ...
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Gut, Marta
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Heath, Simon
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Gut, Ivo Glynne
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Wallentin, Lars
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Gustafsson, Mats ...
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Kamali-Moghaddam ...
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Landegren, Ulf
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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PLOS ONE
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Uppsala University