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  • Manning, Gerard (författare)

The minimal kinome of Giardia lamblia illuminates early kinase evolution and unique parasite biology

  • Artikel/kapitelEngelska2011

Förlag, utgivningsår, omfång ...

  • 2011-07-25
  • Springer Science and Business Media LLC,2011
  • electronicrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:uu-163258
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-163258URI
  • https://doi.org/10.1186/gb-2011-12-7-r66DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:123555947URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Background: The major human intestinal pathogen Giardia lamblia is a very early branching eukaryote with a minimal genome of broad evolutionary and biological interest. Results: To explore early kinase evolution and regulation of Giardia biology, we cataloged the kinomes of three sequenced strains. Comparison with published kinomes and those of the excavates Trichomonas vaginalis and Leishmania major shows that Giardia's 80 core kinases constitute the smallest known core kinome of any eukaryote that can be grown in pure culture, reflecting both its early origin and secondary gene loss. Kinase losses in DNA repair, mitochondrial function, transcription, splicing, and stress response reflect this reduced genome, while the presence of other kinases helps define the kinome of the last common eukaryotic ancestor. Immunofluorescence analysis shows abundant phospho-staining in trophozoites, with phosphotyrosine abundant in the nuclei and phosphothreonine and phosphoserine in distinct cytoskeletal organelles. The Nek kinase family has been massively expanded, accounting for 198 of the 278 protein kinases in Giardia. Most Neks are catalytically inactive, have very divergent sequences and undergo extensive duplication and loss between strains. Many Neks are highly induced during development. We localized four catalytically active Neks to distinct parts of the cytoskeleton and one inactive Nek to the cytoplasm. Conclusions: The reduced kinome of Giardia sheds new light on early kinase evolution, and its highly divergent sequences add to the definition of individual kinase families as well as offering specific drug targets. Giardia's massive Nek expansion may reflect its distinctive lifestyle, biphasic life cycle and complex cytoskeleton.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Reiner, David S. (författare)
  • Lauwaet, Tineke (författare)
  • Dacre, Michael (författare)
  • Smith, Alias (författare)
  • Zhai, Yufeng (författare)
  • Svärd, StaffanUppsala universitet,Mikrobiologi(Swepub:uu)stsva021 (författare)
  • Gillin, Frances D. (författare)
  • Uppsala universitetMikrobiologi (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Genome Biology: Springer Science and Business Media LLC12:7, s. R66-1465-69061474-760X

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