Sökning: L773:1095 9130 OR L773:1046 2023
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A strategy for iden...
A strategy for identification of protein tyrosine phosphorylation
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- Bergström Lind, Sara (författare)
- Uppsala universitet,Genomik
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- Artemenko, Konstantin A. (författare)
- Uppsala universitet,Analytisk kemi
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- Pettersson, Ulf (författare)
- Uppsala universitet,Genomik
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(creator_code:org_t)
- Elsevier BV, 2012
- 2012
- Engelska.
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Ingår i: Methods. - : Elsevier BV. - 1046-2023 .- 1095-9130. ; 56:2, s. 275-283
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- To develop methods for studying phosphorylation of protein tyrosine residues is an important task since this protein modification regulates many cellular functions and often is involved in oncogenesis. An optimal protocol includes enrichment of tyrosine phosphorylated (pTyr) peptides or proteins, followed by a high resolving analytical method for identification of the enriched components. In this Methods paper, we describe a working strategy on how immunoaffinity enrichments, using anti-pTyr antibodies, combined with mass spectrometric (MS) analysis can be used to study the pTyr proteome. We describe in detail how our procedure was used to characterize the pTyr proteome of K562 leukemia cells. Important questions concerning the use of different anti-pTyr antibodies, enrichments performed at the peptide and/or the protein level, pooling of enrichments and requirements for the MS characterization are discussed.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- microdialysis
- ultrafiltration
- free fraction
- ropivacaine
- capillary liquid chromatography
- PROTEIN-BINDING
- MASS-SPECTROMETRY
- BLOOD-PLASMA
- PHARMACOKINETICS
- BUPIVACAINE
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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