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Simultaneous genoty...
Simultaneous genotyping of all hemagglutinin and neuraminidase subtypes of avian influenza viruses by use of padlock probes
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Gyarmati, Péter (author)
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- Conze, Tim (author)
- Uppsala universitet,Molekylära verktyg
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Zohari, Siamak (author)
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LeBlanc, Neil (author)
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- Nilsson, Mats (author)
- Uppsala universitet,Molekylära verktyg
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- Landegren, Ulf (author)
- Uppsala universitet,Molekylära verktyg
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Banér, Johan (author)
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Belák, Sándor (author)
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(creator_code:org_t)
- 2008
- 2008
- English.
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In: Journal of Clinical Microbiology. - 0095-1137 .- 1098-660X. ; 46:5, s. 1747-1751
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- A subtyping assay for both the hemagglutinin (HA) and neuraminidase (NA) surface antigens of the avian influenza virus (AIV) has been developed. The method uses padlock probe chemistry combined with a microarray output for detection. The outstanding feature of this assay is its capability to designate both the HA and the NA of an AIV sample from a single reaction mixture. A panel of 77 influenza virus strains was tested representing the entire assortment of the two antigens. One hundred percent (77/77) of the samples tested were identified as AIV, and 97% (75/77) were subtyped correctly in accordance with previous examinations performed by classical diagnostic methods. Testing of heterologous pathogens verified the specificity of the assay. This assay is a convenient and practical tool for the study of AIVs, providing important HA and NA data more rapidly than conventional methods.
Keyword
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
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