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- Gyarmati, Péter (author)
Simultaneous genotyping of all hemagglutinin and neuraminidase subtypes of avian influenza viruses by use of padlock probes
- Article/chapterEnglish2008
Publisher, publication year, extent ...
- 2008
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:uu-16412
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-16412URI
- https://doi.org/10.1128/JCM.02292-07DOI
Supplementary language notes
- Language:English
- Summary in:English
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- SwePubSwePub
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Notes
- A subtyping assay for both the hemagglutinin (HA) and neuraminidase (NA) surface antigens of the avian influenza virus (AIV) has been developed. The method uses padlock probe chemistry combined with a microarray output for detection. The outstanding feature of this assay is its capability to designate both the HA and the NA of an AIV sample from a single reaction mixture. A panel of 77 influenza virus strains was tested representing the entire assortment of the two antigens. One hundred percent (77/77) of the samples tested were identified as AIV, and 97% (75/77) were subtyped correctly in accordance with previous examinations performed by classical diagnostic methods. Testing of heterologous pathogens verified the specificity of the assay. This assay is a convenient and practical tool for the study of AIVs, providing important HA and NA data more rapidly than conventional methods.
Subject headings and genre
- MEDICINE
- MEDICIN
Added entries (persons, corporate bodies, meetings, titles ...)
- Conze, TimUppsala universitet,Molekylära verktyg(Swepub:uu)ticon367 (author)
- Zohari, Siamak (author)
- LeBlanc, Neil (author)
- Nilsson, MatsUppsala universitet,Molekylära verktyg(Swepub:uu)matsnils (author)
- Landegren, UlfUppsala universitet,Molekylära verktyg(Swepub:uu)ulfland (author)
- Banér, Johan (author)
- Belák, Sándor (author)
- Uppsala universitetMolekylära verktyg (creator_code:org_t)
Related titles
- In:Journal of Clinical Microbiology46:5, s. 1747-17510095-11371098-660X
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- Banér, Johan
- Belák, Sándor
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