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Polypeptide Folding...
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Aili, DanielUniversity of London Imperial College of Science Technology and Medicine
(författare)
Polypeptide Folding-Mediated Tuning of the Optical and Structural Properties of Gold Nanoparticle Assemblies
- Artikel/kapitelEngelska2011
Förlag, utgivningsår, omfång ...
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2011-11-08
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American Chemical Society (ACS),2011
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-166733
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-166733URI
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https://doi.org/10.1021/nl203559sDOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-73736URI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Responsive hybrid nanomaterials with well-defined properties are of significant interest for the development of biosensors with additional applications in tissue engineering and drug delivery. Here, we present a detailed characterization using UV-vis spectroscopy and small angle X-ray scattering of a hybrid material comprised of polypeptide-decorated gold nanoparticles with highly controllable assembly properties. The assembly is triggered by a folding-dependent bridging of the particles mediated by the heteroassociation of immobilized helix-loop-helix polypeptides and a complementary nonlinear polypeptide present in solution. The polypeptides are de novo designed to associate and fold into a heterotrimeric complex comprised of two disulfide-linked four-helix bundles. The particles form structured assemblies with a highly defined interparticle gap (4.8 +/- 0.4 nm) that correlates to the size of the folded polypeptides. Transitions in particle aggregation dynamics, mass-fractal dimensions and ordering, as a function of particle size and the concentration of the bridging polypeptide, are observed; these have significant effects on the optical properties of the assemblies. The assembly and ordering of the particles are highly complex processes that are affected by a large number of variables including the number of polypeptides bridging the particles and the particle mobility within the aggregates. A fundamental understanding of these processes is of paramount interest for the development of novel hybrid nanomaterials with tunable structural and optical properties and for the optimization of nanoparticle-based colorimetric biodetection strategies.
Ämnesord och genrebeteckningar
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Gold nanoparticles
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polypeptides
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folding
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helix-loop-helix
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small angle X-ray scattering (SAXS)
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MEDICINE
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Gryko, PiotrUniversity of London Imperial College of Science Technology and Medicine
(författare)
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Sepulveda, BorjaResearch Centre Nanosci and Nanotechnol CIN2 CSIC
(författare)
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Dick, John A. G.University of London Imperial College of Science Technology and Medicine
(författare)
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Kirby, NigelAustralian Synchrotron
(författare)
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Heenan, RichardRutherford Appleton Lab
(författare)
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Baltzer, LarsUppsala universitet,Institutionen för biokemi och organisk kemi,Uppsala University(Swepub:uu)labal087
(författare)
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Liedberg, BoLinköpings universitet,Sensorvetenskap och Molekylfysik,Tekniska högskolan(Swepub:liu)boli31
(författare)
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Ryan, Mary P.University of London Imperial College of Science Technology and Medicine
(författare)
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Stevens, Molly M.University of London Imperial College of Science Technology and Medicine
(författare)
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University of London Imperial College of Science Technology and MedicineResearch Centre Nanosci and Nanotechnol CIN2 CSIC
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Nano letters (Print): American Chemical Society (ACS)11:12, s. 5564-55731530-69841530-6992
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- Av författaren/redakt...
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Aili, Daniel
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Gryko, Piotr
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Sepulveda, Borja
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Dick, John A. G.
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Kirby, Nigel
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Heenan, Richard
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visa fler...
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Baltzer, Lars
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Liedberg, Bo
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Ryan, Mary P.
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Stevens, Molly M ...
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visa färre...
- Artiklar i publikationen
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Nano letters (Pr ...
- Av lärosätet
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Uppsala universitet
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Linköpings universitet