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- Humble, ElisabetUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,engström lorentz (author)
Amino acid sequence at the phosphorylated site of rat liver fructose-1,6-diphosphatase and phosphorylation of a corresponding synthetic peptide
- Article/chapterEnglish1979
Publisher, publication year, extent ...
- 1979
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:uu-169296
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-169296URI
- https://doi.org/10.1016/0006-291X(79)91934-XDOI
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- Language:English
- Summary in:English
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Notes
- Rat liver fructose-1,6-diphosphatase was phosphorylated with (32P)ATP and the catalytic subunit of cyclic AMP-dependent protein kinase from pig muscle. After digestion with pepsin, α-chymotrypsin and subtilisin a peptide with the amino-terminal sequence Ser-Arg-Tyr-(32P)SerP-Leu-Pro-Leu-Pro was isolated. A synthetic unphosphorylated heptapeptide with the same amino acid sequence, ending with leucine, was phosphorylated with an apparent Km of 400 μM, while the apparent Km value for fructose-1,6-diphosphatase was 30 μM (subunit concentration). The Vmax value was 20 times higher for the peptide than for the enzyme.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Annan medicin och hälsovetenskap hsv//swe
- MEDICAL AND HEALTH SCIENCES Other Medical and Health Sciences hsv//eng
- MEDICIN OCH HÄLSOVETENSKAP Annan medicin och hälsovetenskap Övrig annan medicin och hälsovetenskap hsv//swe
- MEDICAL AND HEALTH SCIENCES Other Medical and Health Sciences Other Medical and Health Sciences not elsewhere specified hsv//eng
Added entries (persons, corporate bodies, meetings, titles ...)
- Dahlqvist-Edberg, UllaUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,engström lorentz (author)
- Ek, PiaUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,engström lorentz(Swepub:uu)pek21046 (author)
- Netzel, ElvyUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,engström lorentz (author)
- Ragnarsson, UlfUppsala universitet,Biokemi,Institutionen för naturvetenskaplig biokemi,ulf ragnarsson (author)
- Engström, LorentzUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,engström lorentz (author)
- Uppsala universitetInstitutionen för medicinsk biokemi och mikrobiologi (creator_code:org_t)
Related titles
- In:Biochemical and Biophysical Research Communications - BBRC90:3, s. 1064-10720006-291X1090-2104
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