Time-resolved protein nanocrystallography using an X-ray free-electron laser

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Aquila, Andrew (author)

Time-resolved protein nanocrystallography using an X-ray free-electron laser

Article/chapterEnglish2012

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2012
printrdacarrier

Numbers

LIBRIS-ID:oai:DiVA.org:uu-169660
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-169660URI
https://doi.org/10.1364/OE.20.002706DOI
https://gup.ub.gu.se/publication/158025URI

Supplementary language notes

Language:English
Summary in:English

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SwePubSwePub

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Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

We demonstrate the use of an X-ray free electron laser synchronized with an optical pump laser to obtain X-ray diffraction snapshots from the photoactivated states of large membrane protein complexes in the form of nanocrystals flowing in a liquid jet. Light-induced changes of Photosystem I-Ferredoxin co-crystals were observed at time delays of 5 to 10 µs after excitation. The result correlates with the microsecond kinetics of electron transfer from Photosystem I to ferredoxin. The undocking process that follows the electron transfer leads to large rearrangements in the crystals that will terminally lead to the disintegration of the crystals. We describe the experimental setup and obtain the first time-resolved femtosecond serial X-ray crystallography results from an irreversible photo-chemical reaction at the Linac Coherent Light Source. This technique opens the door to time-resolved structural studies of reaction dynamics in biological systems.

Subject headings and genre

NATURVETENSKAP Biologi Strukturbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Structural Biology hsv//eng
NATURVETENSKAP Biologi Biofysik hsv//swe
NATURAL SCIENCES Biological Sciences Biophysics hsv//eng
NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
Laser-induced chemistry
Ultrafast lasers
Medical optics and biotechnology
Ultrafast technology
X-ray imaging

Added entries (persons, corporate bodies, meetings, titles ...)

Hunter, Mark S. (author)
Doak, R. Bruce (author)
Kirian, Richard A. (author)
Fromme, Petra (author)
White, Thomas A. (author)
Andreasson, JakobUppsala universitet,Molekylär biofysik(Swepub:uu)jakan998 (author)
Arnlund, DavidGothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xarnda (author)
Bajt, Saša (author)
Barends, Thomas R. M. (author)
Barthelmess, Miriam (author)
Bogan, Michael J. (author)
Bostedt, Christoph (author)
Bottin, Hervé (author)
Bozek, John D. (author)
Caleman, CarlUppsala universitet,Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Molekylär biofysik(Swepub:uu)fimai103 (author)
Coppola, Nicola (author)
Davidsson, JanUppsala universitet,Fysikalisk kemi(Swepub:uu)jandavid (author)
DePonte, Daniel P. (author)
Elser, Veit (author)
Epp, Sascha W. (author)
Erk, Benjamin (author)
Fleckenstein, Holger (author)
Foucar, Lutz (author)
Frank, Matthias (author)
Fromme, Raimund (author)
Graafsma, Heinz (author)
Grotjohann, Ingo (author)
Gumprecht, Lars (author)
Hajdu, JanosUppsala universitet,Molekylär biofysik(Swepub:uu)janohajd (author)
Hampton, Christina Y. (author)
Hartmann, Andreas (author)
Hartmann, Robert (author)
Hau-Riege, Stefan (author)
Hauser, Günter (author)
Hirsemann, Helmut (author)
Holl, Peter (author)
Holton, James M. (author)
Hömke, André (author)
Johansson, Linda (author)
Kimmel, Nils (author)
Kassemeyer, Stephan (author)
Krasniqi, Faton (author)
Kühnel, Kai-Uwe (author)
Liang, Mengning (author)
Lomb, Lukas (author)
Malmerberg, Erik,1980Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xmaler (author)
Marchesini, Stefano (author)
Martin, Andrew V. (author)
Maia, Filipe R.N.C.Gothenburg University,Göteborgs universitet,Uppsala universitet,Molekylär biofysik,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xneuri (author)
Seibert, M. MarvinUppsala universitet,Molekylär biofysik(Swepub:uu)masei255 (author)
Nass, Karol (author)
Reich, Christian (author)
Neutze, Richard (author)
Rolles, Daniel (author)
Rudek, Benedikt (author)
Rudenko, Artem (author)
Schlichting, Ilme (author)
Schmidt, Carlo (author)
Schmidt, Kevin E. (author)
Schulz, Joachim (author)
Seibert, M. MarvinUppsala universitet,Molekylär biofysik(Swepub:uu)masei255 (author)
Soltau, Heike (author)
Shoeman, Robert L. (author)
Sierra, Raymond (author)
Starodub, Dmitri (author)
Stellato, Francesco (author)
Stern, Stephan (author)
Strüder, Lothar (author)
Timneanu, NicusorUppsala universitet,Molekylär biofysik(Swepub:uu)nti19847 (author)
Ullrich, Joachim (author)
Wang, Xiaoyu (author)
Williams, Garth J. (author)
Weidenspointner, Georg (author)
Weierstall, Uwe (author)
Wunderer, Cornelia (author)
Barty, Anton (author)
Spence, John C. H. (author)
Chapman, Henry N. (author)
Uppsala universitetMolekylär biofysik (creator_code:org_t)

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In:Optics Express20:3, s. 2706-27161094-4087

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By the author/editor: Aquila, Andrew; Hunter, Mark S.; Doak, R. Bruce; Kirian, Richard ...; Fromme, Petra; White, Thomas A.; show more...; Andreasson, Jako ...; Arnlund, David; Bajt, Saša; Barends, Thomas ...; Barthelmess, Mir ...; Bogan, Michael J ...; Bostedt, Christo ...; Bottin, Hervé; Bozek, John D.; Caleman, Carl; Coppola, Nicola; Davidsson, Jan; DePonte, Daniel ...; Elser, Veit; Epp, Sascha W.; Erk, Benjamin; Fleckenstein, Ho ...; Foucar, Lutz; Frank, Matthias; Fromme, Raimund; Graafsma, Heinz; Grotjohann, Ingo; Gumprecht, Lars; Hajdu, Janos; Hampton, Christi ...; Hartmann, Andrea ...; Hartmann, Robert; Hau-Riege, Stefa ...; Hauser, Günter; Hirsemann, Helmu ...; Holl, Peter; Holton, James M.; Hömke, André; Johansson, Linda; Kimmel, Nils; Kassemeyer, Step ...; Krasniqi, Faton; Kühnel, Kai-Uwe; Liang, Mengning; Lomb, Lukas; Malmerberg, Erik ...; Marchesini, Stef ...; Martin, Andrew V ...; Maia, Filipe R.N ...; Seibert, M. Marv ...; Nass, Karol; Reich, Christian; Neutze, Richard; Rolles, Daniel; Rudek, Benedikt; Rudenko, Artem; Schlichting, Ilm ...; Schmidt, Carlo; Schmidt, Kevin E ...; Schulz, Joachim; Soltau, Heike; Shoeman, Robert ...; Sierra, Raymond; Starodub, Dmitri; Stellato, France ...; Stern, Stephan; Strüder, Lothar; Timneanu, Nicuso ...; Ullrich, Joachim; Wang, Xiaoyu; Williams, Garth ...; Weidenspointner, ...; Weierstall, Uwe; Wunderer, Cornel ...; Barty, Anton; Spence, John C. ...; Chapman, Henry N ...; show less...

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NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Structural Biolo ...

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biophysics

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

Articles in the publication: Optics Express

By the university: Uppsala University; University of Gothenburg

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