Low Charge Density Cationic Polymers for Gene Delivery : Exploring the Influence of Structural Elements on In Vitro Transfection

• A series of end-functionalized poly(trimethylene carbonate) DNA carriers, characterized by low cationic charge density and pronounced hydrophobicity, was used to study structural effects on in vitro gene delivery. As the DNA-binding moieties were identical in all polymer structures, the differences observed between the different polymers were directly related to the functionality and length of the polymer backbone. The transfection efficiency and cytotoxicity of the polymer/DNA complexes were thus found to be dependent on a combination of polymer charge density and functionality, highlighting the importance of such structural considerations in the development of materials for efficient gene delivery.

Ämnesord

NATURVETENSKAP  -- Kemi -- Polymerkemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Polymer Chemistry (hsv//eng)

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

amphiphiles

biodegradable

biological applications of polymers

gene delivery

transfection

Polymer chemistry

Polymerkemi

Chemistry with specialization in Polymer Chemistry

Kemi med inriktning mot polymerkemi

Publikations- och innehållstyp

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