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Microsomal Glutathi...
Microsomal Glutathione Transferase 1 Protects Against Toxicity Induced by Silica Nanoparticles but Not by Zinc Oxide Nanoparticles
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- Shi, Jingwen (author)
- Institute of Environmental Medicine, KI, Stockholm
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- Karlsson, Hanna L. (author)
- Karolinska Institutet,Institute of Environmental Medicine, KI, Stockholm
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- Johansson, Katarina (author)
- Karolinska Institutet,Institute of Environmental Medicine, KI, Stockholm
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- Gogvadze, Vladimir (author)
- Karolinska Institutet,Institute of Environmental Medicine, KI, Stockholm
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- Xiao, Lisong (author)
- Inorganic and Materials Chemistry, University of Cologne, Köln, Tyskland
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- Li, Jiangtian (author)
- Inorganic and Materials Chemistry, University of Cologne, Köln, Tyskland
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- Burks, Terrance (author)
- KTH,Funktionella material, FNM,Functional Materials Microelectronics and Applied Physics, School of Information and Communication Technology, KTH, Kista
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- Garcia-Bennett, Alfonso (author)
- Uppsala universitet,Nanoteknologi och funktionella material
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- Uheida, Abdusalam (author)
- KTH,Funktionella material, FNM,Functional Materials Microelectronics and Applied Physics, School of Information and Communication Technology, KTH, Kista
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- Muhammed, Mamoun (author)
- KTH,Funktionella material, FNM,Functional Materials Microelectronics and Applied Physics, School of Information and Communication Technology, KTH, Kista
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- Mathur, Sanjay (author)
- Inorganic and Materials Chemistry, University of Cologne, Köln, Tyskland
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- Morgenstern, Ralf (author)
- Karolinska Institutet,Institute of Environmental Medicine, KI, Stockholm
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- Kagan, Valerian E. (author)
- Dept of Environmental and Occupational Health, University of Pittsburgh, PA, USA
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- Fadeel, Bengt (author)
- Karolinska Institutet,Institute of Environmental Medicine, KI, Stockholm
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(creator_code:org_t)
- 2012-02-13
- 2012
- English.
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In: ACS Nano. - : American Chemical Society (ACS). - 1936-0851 .- 1936-086X. ; 6:3, s. 1925-1938
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Abstract
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- Microsomal glutathione transferase 1 (MGST1) is an antioxidant enzyme located predominantly in the mitochondrial er membrane and endoplasmk reticulum and has been shown to protect cells from lipid peroxidation induced by a variety of cytostatic drugs and pro-oxidant stimuli. We hypothesized that MGST1 may also protect against nanomaterial-induced cytotoxicity through a specific effect on lipid peroxidation. We evaluated the induction of cytotoxicity and oxidative stress by TiO2, CeO2, SiO2, and ZnO in the human MCF-7 cell line with or without overexpression of MGST1. SiO2 and ZnO nanoparticles caused dose- and time-dependent toxicity, whereas no obvious cytotoxic effects were induced by nanoparticles of TiO2 and CeO2. We also noted pronounced cytotoxicity for three out of four additional SiO2 nanoparticles tested. Overexpression of MGST1 reversed the cytotoxicity of the main SiO2 nanoparticles tested and for one of the supplementary SiO2 nanoparticles but did not protect cells against ZnO-induced cytotoxic effects. The data point toward a role of lipid peroxidation In SiO2 nanoparticle-induced cell death. For ZnO nanoparticles, rapid dissolution was observed, and the subsequent interaction of Zn2+ with cellular targets is likely to contribute to the cytotoxic effects. A direct inhibition of MGST1 by Zn2+ could provide a possible explanation for the lack of protection against ZnO nanoparticles in this model. Our data also showed that SiO2 nanoparticle-induced cytotoxicity is mitigated in the presence of serum, potentially through masking of reactive surface groups by serum proteins, whereas ZnO nanoparticles were cytotoxic both In the presence and in the absence of serum.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Nanoteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Nano-technology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Materialteknik -- Annan materialteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Materials Engineering -- Other Materials Engineering (hsv//eng)
Keyword
- engineered nanoparticles
- oxidative stress
- lipid peroxidation
- microsomal glutathione transferase 1
- TECHNOLOGY
- TEKNIKVETENSKAP
- Teknisk fysik med inriktning mot nanoteknologi och funktionella material
- Engineering Science with specialization in Nanotechnology and Functional Materials
Publication and Content Type
- ref (subject category)
- art (subject category)
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ACS Nano
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Shi, Jingwen
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Karlsson, Hanna ...
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Johansson, Katar ...
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Gogvadze, Vladim ...
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Xiao, Lisong
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Li, Jiangtian
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Burks, Terrance
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Garcia-Bennett, ...
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Uheida, Abdusala ...
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Muhammed, Mamoun
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Mathur, Sanjay
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Morgenstern, Ral ...
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Kagan, Valerian ...
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Fadeel, Bengt
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- ENGINEERING AND TECHNOLOGY
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and Nano technology
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ENGINEERING AND ...
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ACS Nano
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Uppsala University
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Royal Institute of Technology
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Karolinska Institutet