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Three-dimensional structure determination with an X-ray laser

Ekeberg, Tomas, 1983- (author)
Uppsala universitet,Molekylär biofysik,Molecular biophysics
Svenda, Martin (author)
Abergel, Chantal (author)
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Maia, Filipe (author)
Seltzer, Virginie (author)
Hantke, Max (author)
De Ponte, Daniel (author)
Aquila, Andrew (author)
Schulz, Joachim (author)
Andreasson, Jakob (author)
Iwan, Bianca (author)
Jönsson, Olof (author)
Westphal, Daniel (author)
Odić, Duško (author)
Andersson, Inger (author)
Barty, Anton (author)
Liang, Meng (author)
Seiberg, Marvin (author)
Martin, Andrew (author)
Nass, Karol (author)
Wang, Fenglin (author)
White, Thomas (author)
Gumprecht, Lars (author)
Fleckenstein, Holger (author)
Bajt, Saša (author)
Barthelmess, Miriam (author)
Claverie, Jean-Michel (author)
Tegze, Miklos (author)
Bortel, Gabor (author)
Faigel, Gyula (author)
Loh, Ne-Te Duane (author)
Coppola, Nicola (author)
Bostedt, Christoph (author)
Bozek, John (author)
Krzywinski, Jacek (author)
Messerschmidt, Marc (author)
Hodgson, Keith (author)
Treusch, Rolf (author)
Toleikis, Sven (author)
Dusterer, Stefan (author)
Feldhaus, Josef (author)
Weckert, Edgar (author)
Bogan, Michael (author)
Hampton, Christina (author)
Sierra, Raymond (author)
Doak, Bruce (author)
Weierstall, Uwe (author)
Spence, John (author)
Frank, Matthias (author)
Shoeman, Robert (author)
Lomb, Lukas (author)
Foucar, Lutz (author)
Epp, Sascha (author)
Rolles, Daniel (author)
Rudenko, Artem (author)
Hartmann, Robert (author)
Hartmann, Andreas (author)
Kimmel, Nils (author)
Holl, Peter (author)
Rudek, Benedikt (author)
Erk, Benjamin (author)
Kassemeyer, Stephan (author)
Schlichting, Ilme (author)
Strüder, Lothar (author)
Ullrich, Joachim (author)
Schmidt, Carlo (author)
Krasniqi, Faton (author)
Hauser, Günter (author)
Reich, Christian (author)
Soltau, Heike (author)
Schorb, Sebastian (author)
Hirsemann, Helmut (author)
Wunderer, Cornelia (author)
Graafsma, Heinz (author)
Chapman, Henry (author)
Hajdu, Janos (author)
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 (creator_code:org_t)
English.
  • Other publication (other academic/artistic)
Abstract Subject headings
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  • Three-dimensional structure determination of a non-crystalline virus has been achieved from a set of randomly oriented continuous diffraction patterns captured with an X-ray laser. Intense, ultra-short X-ray pulses intercepted a beam of single mimivirus particles, producing single particle X-ray diffraction patterns that are assembled into a three-dimensional amplitude distribution based on statistical consistency. Phases are directly retrieved from the assembled Fourier distribution to synthesize a three-dimensional image. The resulting electron density reveals a pseudo-icosahedral asymmetric virion structure with a compartmentalized interior, within which the DNA genome occupies only about a fifth of the volume enclosed by the capsid. Additional electron microscopy data indicate the genome has a chromatin-like fiber structure that has not previously been observed in a virus. 

Keyword

Mimivirus
flash diffraction
three dimensional
imaging
CXI

Publication and Content Type

vet (subject category)
ovr (subject category)

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