Smart design of stable hydrazone crosslinked extracellular matrix mimetic hydrogel for tissue engineering application

Injectable hydrogels are important biomaterials with enormous applications. They are used for various biomedical applications such as diagnostics, 3D cell culture matrix, drug reservoir, encapsulation of bioactive compounds and growth factors, scaffold for tissue engineering etc. We here present our recent development in our efforts to develop hydrogel scaffolds with enhanced rigidity, stability, swelling characteristics. Hydrazone crosslinked gels are attractive due to its simplicity and versatility which could be formed by mixing appropriate aldehyde and hydrazide functionalized hyaluronan. By fine-tuning the electronic character around the hydrazone linkage, we succeeded in developing extremely stable hydrazone bond and utilized it for developing hyaluronan (HA) based synthetic extracellular matrix (ECM) hydrogel. Among the different hydrazides tested, we identified carbonyldihydrazide (CDH) as the best candidate to deliver stable hydrazone linkage. This stability is presumably due to extensive delocalization of the positive charge across neighboring amino groups of CDH. The hydrolytic stability imparted by this group was found to be several folds under acidic, basic and physiological pH when compared to other hydrazones. This tailored hydrogel with CDH also exhibited superior swelling and mechanical properties and enzymatic stability which makes it ideal for tissue engineering application.

Wang, ShujiangUppsala universitet,Polymerkemi(Swepub:uu)shuwa138 (författare)
Kisiel, MartaUppsala universitet,Polymerkemi(Swepub:uu)marki526 (författare)
Hilborn, Jöns,1956-Uppsala universitet,Polymerkemi(Swepub:uu)johil255 (författare)
Varghese, Oommen P.,1977-Uppsala universitet,Polymerkemi(Swepub:uu)oopva021 (författare)
Uppsala universitetPolymerkemi (creator_code:org_t)

Ingår i:Journal of Tissue Engineering and Regenerative Medicine: Hindawi Limited6:suppl 1, s. 192-1921932-6254

Av författaren/redakt...: Podiyan, Oommen, ...; Wang, Shujiang; Kisiel, Marta; Hilborn, Jöns, 1 ...; Varghese, Oommen ...

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