A longitudinal study of antimicrobial resistant faecal bacteria in sediments collected from a hospital wastewater system

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Ottoson, JakobSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health (författare)

A longitudinal study of antimicrobial resistant faecal bacteria in sediments collected from a hospital wastewater system

Artikel/kapitelEngelska2012

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2012-03-27
Informa UK Limited,2012
printrdacarrier

Nummerbeteckningar

LIBRIS-ID:oai:DiVA.org:uu-189467
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-189467URI
https://doi.org/10.3402/iee.v2i0.7438DOI
https://res.slu.se/id/publ/38667URI

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Språk:engelska
Sammanfattning på:engelska

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Ämneskategori:ref swepub-contenttype
Ämneskategori:art swepub-publicationtype

Anmärkningar

Objective:The objective with this study was to determine and follow antimicrobial resistance in faecal bacteria over time in hospital wastewater pipe sediment. A further aim was to determine bacterial growth rates of sensitive, intermediate and resistant intestinal enterococci in different ciprofloxacin concentrations as a measure of bacterial fitness.Methods:A system enabling the collection of settled particles over time was installed at Kalmar County Hospital. Samples were collected bi-monthly for a 14-month period. Coliform bacteria and enterococci were isolated from the sediment with standard methods and investigated for resistance to ciprofloxacin (CIP), imipenem (IMI), trimetroprim-sulfamethoxazole (TS), ampicillin (AMP) and vancomycin (VAN) by the disc diffusion method. Resistant isolates were further typed with the PhenePlateTM system. Growth assessments were performed with an automated spectrophotometer.Results:The rate of intestinal enterococci resistance was < 0.6, 1.3, 1.9 and 13% to VAN, IMI, AMP and CIP respectively. Coliform resistance frequencies were 1.1, 2.2 and 2.2% to CIP, IMI and TS respectively. At two sampling occasions, significantly higher rates of ciprofloxacin resistant enterococci were found and the establishment of a resistant clone in the sewer was indicated by the PhP-analysis. Ciprofloxacin resistant intestinal enterococci had a significantly longer lag-phase time than sensitive isolates, but from 500 µg ml-1 (half MIC) resistant isolates had a competitive advantage in terms of significantly faster generation time.Discussion:Despite high concentration of antimicrobials in the sediment, resistance frequencies were generally low. This can depend on limited growth possibilities for faecal bacteria. However, the establishment of a resistant clone shows that hospital sewers can serve as a reservoir for antibiotic resistant bacteria.

Ämnesord och genrebeteckningar

NATURVETENSKAP Biologi Mikrobiologi hsv//swe
NATURAL SCIENCES Biological Sciences Microbiology hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Infektionsmedicin hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Infectious Medicine hsv//eng

Biuppslag (personer, institutioner, konferenser, titlar ...)

Jarnheimer, Per-Åke (författare)
Stenström, Thor Axel (författare)
Olsen, BjörnUppsala universitet,Infektionssjukdomar(Swepub:uu)bjool781 (författare)
Sveriges lantbruksuniversitetInst för biomedicin och veterinär folkhälsovetenskap (creator_code:org_t)
Sveriges lantbruksuniversitet

Sammanhörande titlar

Ingår i:Infection Ecology & Epidemiology: Informa UK Limited2, s. 7438-2000-8686

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Av författaren/redakt...: Ottoson, Jakob; Jarnheimer, Per- ...; Stenström, Thor ...; Olsen, Björn

Om ämnet

NATURVETENSKAP: NATURVETENSKAP; och Biologi; och Mikrobiologi

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Infektionsmedici ...

Artiklar i publikationen: Infection Ecolog ...

Av lärosätet: Uppsala universitet; Sveriges Lantbruksuniversitet

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