Search: id:"swepub:oai:DiVA.org:uu-193252" >
Accounting for stra...
-
Ehrenberg, AngelicaUppsala universitet,Institutionen för kemi - BMC
(author)
Accounting for strain variations and resistance mutations in the characterization of hepatitis C NS3 protease inhibitors
- Article/chapterEnglish2014
Publisher, publication year, extent ...
-
2014-02-11
-
Informa UK Limited,2014
-
printrdacarrier
Numbers
-
LIBRIS-ID:oai:DiVA.org:uu-193252
-
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-193252URI
-
https://doi.org/10.3109/14756366.2013.864651DOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
Context: Natural strain variation and rapid resistance development makes development of broad spectrum hepatitis C virus (HCV) drugs very challenging and evaluation of inhibitor selectivity and resistance must account for differences in the catalytic properties of enzyme variants.Objective: To understand how to study selectivity and relationships between efficacy and genotype or resistant mutants for NS3 protease inhibitors.Materials and methods: The catalytic properties of NS3 protease from genotypes 1a, 1b and 3a, and their sensitivities to four structurally and mechanistically different NS3 protease inhibitors have been analysed under different experimental conditions.Results: The optimisation of buffer conditions for each protease variant enabled the comparison of their catalytic properties and sensitivities to the inhibitors. All inhibitors were most effective against genotype 1a protease, with VX-950 having the broadest selectivity.Discussion and conclusion: A new strategy for evaluation of inhibitors relevant for the discovery of broad spectrum HCV drugs was established.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Schmuck, BenjaminUppsala universitet,Institutionen för kemi - BMC
(author)
-
Anwar, Muhammad IkramUppsala universitet,Institutionen för kemi - BMC
(author)
-
Svahn Gustafsson, SofiaUppsala universitet,Institutionen för kemi - BMC(Swepub:uu)sofsv946
(author)
-
Stenberg, GunUppsala universitet,Institutionen för kemi - BMC
(author)
-
Danielson, HelenaUppsala universitet,Institutionen för kemi - BMC(Swepub:uu)helenads
(author)
-
Uppsala universitetInstitutionen för kemi - BMC
(creator_code:org_t)
Related titles
-
In:Journal of enzyme inhibition and medicinal chemistry (Print): Informa UK Limited29:6, s. 868-8761475-63661475-6374
Internet link
Find in a library
To the university's database