Genes regulating tight junctions and cell adhesion are altered in early experimental necrotizing enterocolitis

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Högberg, Niclas,1979-Uppsala universitet,Barnkirurgi (author)

Genes regulating tight junctions and cell adhesion are altered in early experimental necrotizing enterocolitis

Article/chapterEnglish2013

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Elsevier BV,2013
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LIBRIS-ID:oai:DiVA.org:uu-197750
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-197750URI
https://doi.org/10.1016/j.jpedsurg.2013.06.027DOI

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Language:English
Summary in:English

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De två sista författarna delar sistaförfattarskapet.
Background/purpose:Necrotizing enterocolitis (NEC) represents one of the gravest complications in preterm infants and carries significant morbidity and mortality. Increased intestinal permeability may play an important role in the pathogenesis of NEC. In this study we investigated the genes regulating structural proteins such as tight junctions (TJ) and cell adhesion in a neonatal rat model of early NEC, as well as the expression of TJ proteins by immunohistochemistry staining.Methods:The studies were performed on Sprague-Dawley rat pups. Experimental NEC was induced using hypoxia/reoxygenation treatment on day 1 after birth. Intestinal specimens from the ileum were obtained, mRNA was purified and the transcriptome was analyzed using microarray. Immunohistochemistry staining was performed for TJ proteins.Results:We found several TJ genes such as claudins 1, 8, 14, 15 and gap junction protein to be affected. Immunohistochemistry staining for TJ protein claudin-1 revealed decreased levels in experimental NEC compared to controls. Alterations in genes involved in the inflammatory response was confirmed, along with several genes regulating proteins used as biomarkers for NEC.Conclusion:This study indicates that tight junctions and cell adhesion may play a critical role in the pathogenesis of early experimental NEC. Better understanding of the pathogenesis of NEC may lead to novel strategies for the prevention and treatment of NEC.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Kirurgi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Surgery hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine hsv//eng
Kirurgi, särskilt barnkirurgi
Pediatric Surgery
Pathology
Patologi

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Stenbäck, AndersUppsala universitet,Barnkirurgi(Swepub:uu)andesten (author)
Carlsson, Per-OlaUppsala universitet,Institutionen för medicinsk cellbiologi,Transplantation och regenerativ medicin(Swepub:uu)perocarl (author)
Wanders, AlkwinUppsala universitet,Molekylär och morfologisk patologi(Swepub:uu)alkwwand (author)
Engstrand Lilja, HeléneUppsala universitet,Barnkirurgi(Swepub:uu)helelilj (author)
Uppsala universitetBarnkirurgi (creator_code:org_t)

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In:Journal of Pediatric Surgery: Elsevier BV48:11, s. 2308-23120022-34681531-5037

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