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  • Carlsson, Anja MKarolinska Institutet (författare)

Plasmodium falciparum population dynamics during the early phase of anti-malarial drug treatment in Tanzanian children with acute uncomplicated malaria

  • Artikel/kapitelEngelska2011

Förlag, utgivningsår, omfång ...

  • 2011-12-20
  • Springer Science and Business Media LLC,2011
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:uu-197823
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-197823URI
  • https://doi.org/10.1186/1475-2875-10-380DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:124092580URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • BACKGROUND:This study aimed to explore Plasmodium falciparum population dynamics during the early phase of anti-malarial drug treatment with artemisinin-based combination therapy in children with clinical malaria in a high transmission area in Africa.METHODS:A total of 50 children aged 1-10 years with acute uncomplicated P. falciparum malaria in Bagamoyo District, Tanzania, were enrolled. Participants were hospitalized and received supervised standard treatment with artemether-lumefantrine according to body weight in six doses over 3 days. Blood samples were collected 11 times, i.e. at time of diagnosis (-2 h) and 0, 2, 4, 8, 16, 24, 36, 48, 60 and 72 h after initiation of treatment. Parasite population dynamics were assessed using nested polymerase chain reaction (PCR)-genotyping of merozoite surface protein (msp) 1 and 2.RESULTS:PCR-analyses from nine sequential blood samples collected after initiation of treatment identified 20 and 21 additional genotypes in 15/50 (30%) and 14/50 (28%) children with msp1 and msp2, respectively, non-detectable in the pre-treatment samples (-2 and 0 h combined). Some 15/20 (75%) and 14/21 (67%) of these genotypes were identified within 24 h, whereas 17/20 (85%) and 19/21 (90%) within 48 h for msp1 and msp2, respectively. The genotype profile was diverse, and varied considerably over time both within and between patients, molecular markers and their respective families.CONCLUSION:PCR analyses from multiple blood samples collected during the early treatment phase revealed a complex picture of parasite sub-populations. This underlines the importance of interpreting PCR-outcomes with caution and suggests that the present use of PCR-adjustment from paired blood samples in anti-malarial drug trials may overestimate assessment of drug efficacy in high transmission areas in Africa.The study is registered at http://www.clinicaltrials.gov with identifier NCT00336375.

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Ngasala, Billy E (författare)
  • Dahlström, Sabina (författare)
  • Membi, Christopher (författare)
  • Veiga, Isabel M (författare)
  • Rombo, LarsKarolinska Institutet,Uppsala universitet,Centrum för klinisk forskning i Sörmland (CKFD)(Swepub:uu)larra728 (författare)
  • Abdulla, Salim (författare)
  • Premji, Zul (författare)
  • Gil, J PedroKarolinska Institutet (författare)
  • Björkman, AndersKarolinska Institutet (författare)
  • Mårtensson, Andreas (författare)
  • Karolinska InstitutetCentrum för klinisk forskning i Sörmland (CKFD) (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Malaria Journal: Springer Science and Business Media LLC10, s. 380-1475-2875

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