Dynamic gradients of an intermediate filament-like cytoskeleton are recruited by a polarity landmark during apical growth

Fuchino, KatsuyaLund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Molecular Cell Biology,Department of Biology,Faculty of Science (författare)

Intermediate filament (IF)-like cytoskeleton emerges as a versatile tool for cellular organization in all kingdoms of life, underscoring the importance of mechanistically understanding its diverse manifestations. We showed previously that, in Streptomyces (a bacterium with a mycelial lifestyle similar to that of filamentous fungi, including extreme cell and growth polarity), the IF protein FilP confers rigidity to the hyphae by an unknown mechanism. Here, we provide a possible explanation for the IF-like function of FilP by demonstrating its ability to self-assemble into a cis-interconnected regular network in vitro and its localization into structures consistent with a cytoskeletal network in vivo. Furthermore, we reveal that a spatially restricted interaction between FilP and DivIVA, the main component of the Streptomyces polarisome complex, leads to formation of apical gradients of FilP in hyphae undergoing active tip extension. We propose that the coupling between the mechanism driving polar growth and the assembly of an IF cytoskeleton provides each new hypha with an additional stress-bearing structure at its tip, where the nascent cell wall is inevitably more flexible and compliant while it is being assembled and matured. Our data suggest that recruitment of cytoskeleton around a cell polarity landmark is a broadly conserved strategy in tip-growing cells.

Bagchi, SonchitaUppsala universitet,Institutionen för cell- och molekylärbiologi(Swepub:uu)sobag172 (författare)
Cantlay, StuartLund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Molecular Cell Biology,Department of Biology,Faculty of Science(Swepub:lu)cob-scy (författare)
Sandblad, LindaUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)(Swepub:umu)lisa0088 (författare)
Wu, DiUppsala universitet,Molekylära verktyg,Science for Life Laboratory, SciLifeLab,Landegren(Swepub:uu)diawu946 (författare)
Bergman, JessicaUppsala universitet,Institutionen för cell- och molekylärbiologi(Swepub:uu)jesbe762 (författare)
Kamali-Moghaddam, MasoodUppsala universitet,Molekylära verktyg,Science for Life Laboratory, SciLifeLab,Landegren(Swepub:uu)masookam (författare)
Flärdh, KlasLund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Molecular Cell Biology,Department of Biology,Faculty of Science(Swepub:lu)cob-kfl (författare)
Ausmees, Nora (författare)
Molekylär cellbiologiBiologiska institutionen (creator_code:org_t)

Ingår i:Proceedings of the National Academy of Sciences of the United States of America: Proceedings of the National Academy of Sciences110:21, s. E1889-E18970027-84241091-6490

Av författaren/redakt...: Fuchino, Katsuya; Bagchi, Sonchita; Cantlay, Stuart; Sandblad, Linda; Wu, Di; Bergman, Jessica; visa fler...; Kamali-Moghaddam ...; Flärdh, Klas; Ausmees, Nora; visa färre...

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinska och f ...; och Cell och molekyl ...

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