Effects of di- and polysaccharide formulations and storage conditions on survival of freeze-dried Sphingobium sp.

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Önneby, KarinSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för mikrobiologi,Department of Microbiology,SLU Swedish University of Agricultural Sciences, Sweden (author)

Effects of di- and polysaccharide formulations and storage conditions on survival of freeze-dried Sphingobium sp.

Article/chapterEnglish2013

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2013-03-10
Springer Science and Business Media LLC,2013
printrdacarrier

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LIBRIS-ID:oai:DiVA.org:uu-206581
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-206581URI
https://doi.org/10.1007/s11274-013-1303-7DOI
https://res.slu.se/id/publ/52380URI
https://urn.kb.se/resolve?urn=urn:nbn:se:ri:diva-2410URI

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Language:English
Summary in:English

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SwePubSwePub

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Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

In this study we have compared the ability of the organic polymers Ficoll and hydroxyethylcellulose (HEC) and the disaccharides sucrose and trehalose to support cell survival during freeze-drying and subsequent storage of a gram-negative Sphingobium sp. In addition to determination of viability rates, cell integrity was evaluated using lipid peroxidation and RNA quality assays for the different storage conditions and formulation compositions. All formulations resulted in high initial cell survival rates after freeze-drying. However, the disaccharide formulations were superior to the polymer-based formulations in supporting cell survival during storage with the exception of Ficoll that upon storage under vacuum yielded bacterial survival rates equal to that of sucrose. Storage in the presence of both oxygen and moisture was detrimental for bacterial survival in all formulations tested, however, lipid peroxidation or RNA damages were not the controlling mechanisms for cell death in this system. The ability of Ficoll and HEC to support cell survival during freeze-drying show that organic polymers, expected to lack the water replacing capability of e.g. disaccharides, can successfully be used as lyoprotectants. For storage under vacuum conditions we suggest that the intracellular amount of sugars (i.e. trehalose), or other protective native cell components, is sufficient for a basic protection inside the bacteria cell and that the amorphous state is the most important aspect of the formulation excipient. However, when exposed to oxygen and moisture during storage this protection is not sufficient to prevent cell degeneration.

Subject headings and genre

LANTBRUKSVETENSKAPER Annan lantbruksvetenskap Övrig annan lantbruksvetenskap hsv//swe
AGRICULTURAL SCIENCES Other Agricultural Sciences Other Agricultural Sciences not elsewhere specified hsv//eng
NATURVETENSKAP Biologi Mikrobiologi hsv//swe
NATURAL SCIENCES Biological Sciences Microbiology hsv//eng
LANTBRUKSVETENSKAPER Lantbruksvetenskap, skogsbruk och fiske hsv//swe
AGRICULTURAL SCIENCES Agriculture, Forestry and Fisheries hsv//eng
Freeze-drying
Bacteria
Organic polymers
Disaccharides
Storage stability

Added entries (persons, corporate bodies, meetings, titles ...)

Pizzul, LeticiaRISE,JTI Institutet för Jordbruks- och Miljöteknik(Swepub:slu)47726 (author)
Bjerketorp, JoakimSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för mikrobiologi,Department of Microbiology,SLU Swedish University of Agricultural Sciences, Sweden(Swepub:slu)46873 (author)
Mahlin, DennyUppsala universitet,Institutionen för farmaci,Uppsala University, Sweden(Swepub:uu)dma10394 (author)
Håkansson, SebastianSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för mikrobiologi,Department of Microbiology,SLU Swedish University of Agricultural Sciences, Sweden(Swepub:slu)48672 (author)
Wessman, PerAgResearch,AgResearch, New Zealand(Swepub:slu)47496 (author)
Sveriges lantbruksuniversitetInstitutionen för mikrobiologi (creator_code:org_t)
Sveriges lantbruksuniversitet

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In:World Journal of Microbiology & Biotechnology: Springer Science and Business Media LLC29:8, s. 1399-14080959-39931573-0972

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By the author/editor: Önneby, Karin; Pizzul, Leticia; Bjerketorp, Joak ...; Mahlin, Denny; Håkansson, Sebas ...; Wessman, Per

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