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Enzyme-linked immun...
Enzyme-linked immunosorbent assay using thin-layered microfluidics with perfect capture of the target protein
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- Smirnova, Adelina (författare)
- University of Tokyo
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- Ohta, Ryoichi (författare)
- University of Tokyo
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- Mori, Emi (författare)
- University of Tokyo
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- Shimizu, Hisashi (författare)
- University of Tokyo
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- Morikawa, Kyojiro (författare)
- University of Tokyo,National Tsing Hua University
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- Kitamori, Takehiko (författare)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,LTH profilområde: Teknik för hälsa,LTH profilområden,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,LTH Profile Area: Engineering Health,LTH Profile areas,Faculty of Engineering, LTH,University of Tokyo,National Tsing Hua University
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(creator_code:org_t)
- 2023
- 2023
- Engelska 10 s.
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Ingår i: Analytical Methods. - : Royal Society of Chemistry (RSC). - 1759-9660 .- 1759-9679. ; 15:5, s. 675-684
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We developed a process for enzyme-linked immunosorbent assay on a glass microchip via the use of a thin-layered microfluidic channel. This channel possesses a high aspect ratio (width/depth ∼200) and has an antibody layer immobilized directly on the channel surface. A depth of several microns and an excessive width and length (mm scale) of the channel provide a large-volume capacity (102 nL) and maximum capture efficiency of the analyte for a high level of detection sensitivity (102 pg mL−1). The developed reusable immunosensor has demonstrated high-performance characteristics by requiring less than 50 μL of sample and providing analysis in less than 25 min. This new method could impact the development of point-of-care devices for biomedical applications.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Publikations- och innehållstyp
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- ref (ämneskategori)
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