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Imaging the in vivo...
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Berglund, DavidUppsala universitet,Klinisk immunologi,Transplantationskirurgi
(author)
Imaging the in vivo fate of human T cells following transplantation in immunoincompetent mice - Implications for clinical cell therapy trials
- Article/chapterEnglish2013
Publisher, publication year, extent ...
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Elsevier BV,2013
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-216763
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-216763URI
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https://doi.org/10.1016/j.trim.2013.09.009DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Many forms of adoptive T cell therapy are on the verge of being translated to the clinic. To gain further insight in their immunomodulating functions and to optimize future clinical trials it is essential to develop techniques to study their homing capacity. CD4+ T cells were labeled using [In-111]oxine, and the radioactive uptake was determined in vitro before intravenous injection in immunodeficient mice. In vivo biodistribution of [In-111] oxine-labeled cells or tracer alone was subsequently measured by mu SPECT/CT and organ distribution. CD4+ T cells incorporated [In-111]oxine with higher labeling yield using Ringer-Acetate compared to 0.9% NaCl. Cellular viability after labeling with [In-111]oxine was not compromised using less than 0.4 MBq/million cells. After intravenous infusion CD4+ T cells preferentially homed to the liver (p < 0.01) and spleen (p < 0.05). This study presents a protocol for labeling of T cells by [In-111]oxine with preserved viability and in vivo tracking by SPECT for up to 8 days, which can easily be translated to clinical cell therapy trials.
Subject headings and genre
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T cell
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In vivo imaging
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Cell therapy
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SPECT/CT
Added entries (persons, corporate bodies, meetings, titles ...)
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Karlsson, MarieUppsala universitet,Klinisk immunologi(Swepub:uu)makar254
(author)
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Palanisamy, SenthilkumarUppsala universitet,Plattformen för preklinisk PET
(author)
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Carlsson, BjörnUppsala universitet,Klinisk immunologi
(author)
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Korsgren, OlleUppsala universitet,Klinisk immunologi(Swepub:uu)ollekors
(author)
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Eriksson, OlofUppsala universitet,Plattformen för preklinisk PET(Swepub:uu)oloer586
(author)
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Uppsala universitetKlinisk immunologi
(creator_code:org_t)
Related titles
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In:Transplant Immunology: Elsevier BV29:1-4, s. 105-1080966-32741878-5492
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