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The production of t...
The production of the eosinophil proteins ECP and EPX/EDN are regulated in a reciprocal manner
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- Jönsson, Ulla-Britt (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper
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- Blom, Kristin (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper
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- Stålenheim, Gunnemar (author)
- Uppsala universitet,Lungmedicin och allergologi
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- Douhan Håkansson, Lena (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper
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- Venge, Per (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper
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(creator_code:org_t)
- 2013-07-30
- 2014
- English.
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In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS). - : Wiley. - 0903-4641 .- 1600-0463. ; 122:4, s. 283-291
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Previous studies showed that the biological activity and the eosinophil content of eosinophil cationic protein (ECP, RNase 3) are determined by single-nucleotide polymorphisms (SNPs) in the ECP (RNase3) gene. In this study, we report the prevalence of a common SNP in the eosinophil protein x/eosinophil-derived neurotoxin (EPX/EDN, RNase2) and the association with the cellular contents of EPX/EDN and ECP. The genes were sequenced and the EPX/EDN405(G>C) rs2013109 SNPs were also determined by TaqMan 5nuclease allelic discrimination assay. ECP and EPX/EDN in purified eosinophils or in whole blood extracts were analysed by sensitive immunoassays. The study included 379 non-allergic and allergic subjects. The genotype prevalence of the EPX/EDN405(G>C) polymorphism was GG 59%, GC 36% and CC 6%. The cellular contents of ECP and EPX/EDN were related in a reciprocal fashion with the sums of the protein contents being constant. The contents were associated with the ECP562(G>C) rs2233860 and EPX/EDN405(G>C) gene polymorphisms. The cellular content of eosinophil peroxidase (EPO) was not associated with the ECP and EPX/EDN genotypes. The prevalence of the EPX/EDN405(G>C) genotypes and the contents of the proteins were similar in non-allergic and allergic subjects. The production and storage of the two ancestral proteins, ECP and EPX/EDN likely share common regulatory mechanisms, which result in opposing productions of the two proteins.
Keyword
- Allergy
- eosinophils
- ECP
- eosinophil cationic protein
- EPX
- EDN
- eosinophil protein x
- eosinophil-derived neurotoxin
- genetics
Publication and Content Type
- ref (subject category)
- art (subject category)
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