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Ontogenetic develop...
Ontogenetic development and spatial distribution of the ileal apical sodium-dependent bile acid transporter and the ileal lipid-binding protein in apoE knockout and C57BL/6 mice
- Article/chapterEnglish2002
Publisher, publication year, extent ...
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2009-07-08
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Informa UK Limited,2002
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-227545
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-227545URI
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https://doi.org/10.1080/003655202320378301DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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BACKGROUND: Although apoE-/- mice are characterized by hypercholesterolemia, the bile acid enterohepatic circulation, which plays a crucial role in cholesterol homeostasis, has not been examined in these mice. The differences between apoE-/- and C57BL/6 mice in expression of the ileal ASBT and ILBP and in intestinal bile acid absorption were studied.METHODS: The intestinal tissues of the fetal, neonatal and post-weaning mice were processed for immunohistochemistry. Body retention and fecal excretion of 75SeHCAT were measured. The bile acid pool size and its composition were analysed by HPLC.RESULTS: In apoE-/- and C57BL/6 mice, the bile acid pool size was 75 +/- 13 and 78 +/- 13 micromol/ 100 g body weight, respectively, while the ratio of cholic acid/beta-muricholic acid was 1.8 +/- 0.3 and 1.4 +/- 0.3 (P < 0.05), respectively. The daily body retention of 75SeHCAT was 48% = 1.8% in C57 black mice and 58.4% +/- 2.7% in apoE-/- mice (P < 0.05). In both mouse strains, ASBT expression in the small intestine was found in the near-term fetal and post-weaning mice, while ILBP expression was found in all postnatal mice. In the post-weaning mice, ILBP expression was limited to the distal 25%-30% of the small intestine, while ASBT expression was limited to the distal 18%.CONCLUSIONS: The bile acid enterohepatic circulation in apoE-/- mice probably does not differ greatly from that in C57BL/6 mice.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Andersson, IngelaAstraZeneca R&D Mo¨lndal, Discovery, Mo¨lndal, Sweden(Swepub:uu)ingla753
(author)
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Nyström, S
(author)
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Löfgren, L
(author)
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Amrot, L F
(author)
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Li, Hong
(author)
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AstraZeneca R&D Mo¨lndal, Discovery, Mo¨lndal, Sweden
(creator_code:org_t)
Related titles
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In:Scandinavian Journal of Gastroenterology: Informa UK Limited37:9, s. 1089-10960036-55211502-7708
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