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Autoregulation of t...
Autoregulation of the tufB operon in Salmonella
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- Brandis, Gerrit, 1985- (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Diarmaid Hughes
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- Bergman, Jessica M. (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Diarmaid Hughes
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- Hughes, Diarmaid, 1956- (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Diarmaid Hughes
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(creator_code:org_t)
- 2016-04-05
- 2016
- English.
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In: Molecular Microbiology. - : John Wiley & Sons. - 0950-382X .- 1365-2958. ; 100:6, s. 1004-1016
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Abstract
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- In Salmonella enterica and related species, translation elongation factor EF-Tu is encoded by two widely separated but near-identical genes, tufA and tufB. Two thirds of EF-Tu is expressed from tufA with the remaining one third coming from tufB. Inactivation of tufA is partly compensated by a doubling in the amount of EF-TuB but the mechanism of this up-regulation is unknown. By experimental evolution selecting for improved growth rate in a strain with an inactive tufA we selected six different noncoding or synonymous point mutations close to the tufB start codon. Based on these results we constructed a total of 161 different point mutations around the tufB start codon, as well as tufB 3′-truncations, and measured tufB expression using tufB-yfp transcriptional and translational fusions. The expression data support the presence of two competing stem-loop structures that can form in the 5′-end of the tufB mRNA. Formation of the ‘closed’ structure leads to Rho-dependent transcriptional termination of the tufB mRNA. We propose a model in which translational speed is used as a sensor for EF-Tu concentration and where the expression of tufB is post-transcriptionally regulated. This model describes for the first time how expression of the most abundant Salmonella protein is autoregulated.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- Salmonella enterica
- tufA
- tufB
- EF-Tu
- Rho
- post-transcriptional regulation
- Biologi med inriktning mot mikrobiologi
- Biology with specialization in Microbiology
- Mikrobiologi
- Microbiology
- Molekylär genetik
- Molecular Genetics
Publication and Content Type
- ref (subject category)
- art (subject category)
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