LC/MS at the whole protein level: Studies of biomolecular structure and interactions using native LC/MS and cross-path reactive chromatography (XP-RC) MS

Interfacing liquid chromatography (LC) with electrospray ionization (ESI) to enable on-line MS detection had been initially implemented using reversed phase LC, which in the past three decades remained the default type of chromatography used for LC/MS and LC/MS/MS studies of protein structure. In contrast, the advantages of other types of LC as front-ends for ESI MS, particularly those that allow biopolymer higher order structure to be preserved throughout the separation process, enjoyed relatively little appreciation until recently. However, the past few years witnessed a dramatic surge of interest in the so-called “native” (with “non-denaturing” being perhaps a more appropriate adjective) LC/MS and LC/MS/MS analyses within the bioanalytical and biophysical communities. This review focuses on recent advances in this field, with an emphasis on size exclusion and ion exchange chromatography as front-end platforms for protein characterization by LC/MS. Also discussed are the benefits provided by the integration of chemical reactions in the native LC/MS analyses, including both ion chemistry in the gas phase (e.g., limited charge reduction for characterization of highly heterogeneous biopolymers) and solution-phase reactions (using the recently introduced technique cross-path reactive chromatography).

Pawlowski, Jake W.University of Massachusetts (author)
Yang, WenhuaNanchang University,University of Massachusetts (author)
Muneeruddin, KhajaUniversity of Massachusetts (author)
Yao, HonglinUniversity of Massachusetts (author)
Bobst, Cedric E.University of Massachusetts (author)
Lipatnikov, Andrei,1961Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)lipatn (author)
University of MassachusettsNanchang University (creator_code:org_t)

By the author/editor: Kaltashov, Igor ...; Pawlowski, Jake ...; Yang, Wenhua; Muneeruddin, Kha ...; Yao, Honglin; Bobst, Cedric E.; show more...; Lipatnikov, Andr ...; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

NATURAL SCIENCES: NATURAL SCIENCES; and Chemical Science ...; and Analytical Chemi ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

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