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  • Koos, BjörnUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab (author)

Proximity-dependent initiation of hybridization chain reaction

  • Article/chapterEnglish2015

Publisher, publication year, extent ...

  • 2015-06-12
  • Springer Science and Business Media LLC,2015
  • electronicrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-255596
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-255596URI
  • https://doi.org/10.1038/ncomms8294DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Sensitive detection of protein interactions and post-translational modifications of native proteins is a challenge for research and diagnostic purposes. A method for this, which could be used in point-of-care devices and high-throughput screening, should be reliable, cost effective and robust. To achieve this, here we design a method (proxHCR) that combines the need for proximal binding with hybridization chain reaction (HCR) for signal amplification. When two oligonucleotide hairpins conjugated to antibodies bind in close proximity, they can be activated to reveal an initiator sequence. This starts a chain reaction of hybridization events between a pair of fluorophore-labelled oligonucleotide hairpins, generating a fluorescent product. In conclusion, we show the applicability of the proxHCR method for the detection of protein interactions and posttranslational modifications in microscopy and flow cytometry. As no enzymes are needed, proxHCR may be an inexpensive and robust alternative to proximity ligation assays.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Cane, GaëlleUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab (author)
  • Grannas, KarinUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)kargr691 (author)
  • Löf, LizaUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)lizlo931 (author)
  • Arngården, LindaUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)linan615 (author)
  • Heldin, JohanUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)johel535 (author)
  • Clausson, Carl-MagnusUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)carcl280 (author)
  • Klaesson, AxelUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)axekl423 (author)
  • Hirvonen, M KaroliinaUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab (author)
  • de Oliveira, Felipe Marques SouzaUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)felma546 (author)
  • Talibov, Vladimir OUppsala universitet,Biokemi(Swepub:uu)vlata700 (author)
  • Pham, Nhan TSchool of Biological Sciences and School of Biomedical Sciences, University of Edinburgh, UK (author)
  • Auer, ManfredSchool of Biological Sciences and School of Biomedical Sciences, University of Edinburgh, UK (author)
  • Danielson, U HelenaUppsala universitet,Biokemi(Swepub:uu)helenads (author)
  • Haybaeck, JohannesInstitute of Pathology, Medical University of Graz, Austria (author)
  • Kamali-Moghaddam, MasoodUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)masookam (author)
  • Söderberg, OlaUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)olasoder (author)
  • Uppsala universitetInstitutionen för immunologi, genetik och patologi (creator_code:org_t)

Related titles

  • In:Nature Communications: Springer Science and Business Media LLC62041-1723

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