WFRF:(Dixelius Johan)
Sökning: WFRF:(Dixelius Johan) > (2015-2019) > Plant-mediated gene...
- Jahan, SultanaLinnean Centre for Plant Biology (författare)
Plant-mediated gene silencing restricts growth of the potato late blight pathogen Phytophthora infestans
- Artikel/kapitelEngelska2015
Förlag, utgivningsår, omfång ...
- 2015-03-18
- Oxford University Press (OUP),2015
- electronicrdacarrier
Nummerbeteckningar
- LIBRIS-ID:oai:DiVA.org:uu-256537
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-256537URI
- https://doi.org/10.1093/jxb/erv094DOI
- https://res.slu.se/id/publ/72277URI
Kompletterande språkuppgifter
- Språk:engelska
- Sammanfattning på:engelska
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- SwePubSwePub
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Anmärkningar
- A host-induced gene-silencing strategy for controlling potato late blight is presented, a plant disease that conventionally requires regular application of fungicides at high rates.Phytophthora infestans is an oomycete that causes severe damage to potato, and is well known for its ability to evolve rapidly in order to overcome resistant potato varieties. An RNA silencing strategy was evaluated here to clarify if small interfering RNA homologous to selected genes in P. infestans could be targeted from the plant host to reduce the magnitude of the infection. As a proof-of-concept, a hairpin RNA (hp-RNA) construct using the GFP marker gene was designed and introduced in potato. At 72 hpi, a 55-fold reduction of the signal intensity of a corresponding GFP expressing P. infestans strain on leaf samples of transgenic plants, compared with wild-type potato, was detected. This suggests that an RNA interference construct in the potato host could be processed and target a transcript of the pathogen. Three genes important in the infection process of P. infestans, PiGPB1, PiCESA2, and PiPEC, together with PiGAPDH taking part in basic cell maintenance were subsequently tested using an analogous transgenic strategy. Out of these gene candidates, the hp-PiGPB1 targeting the G protein beta-subunit (PiGPB1) important for pathogenicity resulted in most restricted disease progress. Further, Illumina sequencing of inoculated transgenic potato leaves revealed sRNAs of 24/25 nt size homologous to the PiGPB1 gene in the transgenic plants indicating post-transcriptional silencing of the target gene. The work demonstrates that a host-induced gene-silencing approach is functional against P. infestans but is highly dependent on target gene for a successful outcome. This finding broadens the arsenal of control strategies to this important plant disease.
Ämnesord och genrebeteckningar
- NATURVETENSKAP Biologi hsv//swe
- NATURAL SCIENCES Biological Sciences hsv//eng
- LANTBRUKSVETENSKAPER Lantbruksvetenskap, skogsbruk och fiske Jordbruksvetenskap hsv//swe
- AGRICULTURAL SCIENCES Agriculture, Forestry and Fisheries Agricultural Science hsv//eng
- Late blight
- oomycete
- Phytophthora infestans
- potato
- RNA interference
- small RNA
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Åsman, AnnaLinnean Centre for Plant Biology(Swepub:slu)48014 (författare)
- Corcoran, PadraicUppsala universitet,Systematisk biologi(Swepub:uu)padco251 (författare)
- Fogelqvist, JohanSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology(Swepub:slu)46819 (författare)
- Ramesh, VetukuriLinnean Centre for Plant Biology(Swepub:slu)50600 (författare)
- Dixelius, ChristinaLinnean Centre for Plant Biology(Swepub:slu)48572 (författare)
- Linnean Centre for Plant BiologySystematisk biologi (creator_code:org_t)
- Sveriges lantbruksuniversitet
Sammanhörande titlar
- Ingår i:Journal of Experimental Botany: Oxford University Press (OUP)66:9, s. 2785-27940022-09571460-2431
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- Dixelius, Christ ...
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