Discovery of urinary biomarkers of whole grain rye intake in free-living subjects using non-targeted LC-MS metabolite profiling

Hanhineva, KatiDepartment of Clinical Nutrition, Institute of Public Health and Clinical Nutrition, University of Eastern Finland, Kuopio, Finland (författare)

SCOPE: Whole grain (WG) intake is associated with decreased risk of developing colorectal cancer, type 2 diabetes and cardiovascular disease and its comorbidities. However, the role of specific grains is unclear. Moreover, intake of specific WG is challenging to measure accurately with traditional dietary assessment methods. Our aim was to use non-targeted metabolite profiling to discover specific urinary biomarkers for WG rye to objectively reflect intake under free-living conditions.METHODS AND RESULTS: WG rye intake was estimated by weighed food records, and 24 h urine collections were analyzed by LC-MS. Multivariate modelling was undertaken by repeated double cross-validated partial least squares regression against reported WG rye intake, which correlated well with multivariate prediction estimates (r = 0.67-0.80, p<0.001), but not with intakes of WG wheat or oats. Hydroxyhydroxyphenyl acetamide (HHPA) sulfate, 3,5-dihydroxyphenylpropionic acid (DHPPA) sulfate, caffeic acid sulfate and hydroxyphenyl acetamide (HPAA) sulfate were among the 20 features which had the greatest potential as intake biomarkers of WG. In addition, three compounds exhibited MS/MS fragmentation of carnitine structures.CONCLUSION: With this non-targeted approach, we confirmed the specificity of alkylresorcinol metabolites as biomarkers for WG rye intake, but also discovered other compounds that should be evaluated as putative biomarkers in future studies.

Brunius, CarlSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för livsmedelsvetenskap,Department of Food Science(Swepub:slu)48489 (författare)
Andersson, Agneta,1968-Uppsala universitet,Institutionen för kostvetenskap(Swepub:uu)agnetaand (författare)
Marklund, MattiUppsala universitet,Klinisk nutrition och metabolism,Uppsala University(Swepub:slu)50671 (författare)
Juvonen, RistoSchool of Pharmacy, University of Eastern Finland, Kuopio, Finland (författare)
Keski-Rahkonen, PekkaSchool of Pharmacy, University of Eastern Finland, Kuopio, Finland (författare)
Auriola, SeppoSchool of Pharmacy, University of Eastern Finland, Kuopio, Finland (författare)
Landberg, RikardSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för livsmedelsvetenskap,Department of Food Science,Karolinska Institute(Swepub:slu)51036 (författare)
Department of Clinical Nutrition, Institute of Public Health and Clinical Nutrition, University of Eastern Finland, Kuopio, FinlandInstitutionen för livsmedelsvetenskap (creator_code:org_t)
Sveriges lantbruksuniversitet

Ingår i:Molecular Nutrition & Food Research: Wiley59:11, s. 2315-23251613-41251613-4133

Av författaren/redakt...: Hanhineva, Kati; Brunius, Carl; Andersson, Agnet ...; Marklund, Matti; Juvonen, Risto; Keski-Rahkonen, ...; visa fler...; Auriola, Seppo; Landberg, Rikard; visa färre...

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Hälsovetenskap; och Näringslära

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Hälsovetenskap; och Folkhälsovetensk ...

Av lärosätet: Uppsala universitet; Karolinska Institutet; Sveriges Lantbruksuniversitet

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