Search: L773:0173 0835 OR L773:1522 2683
> (2015-2019) >
Label-free bead-bas...
Label-free bead-based metallothionein electrochemical immunosensor
-
Nejdl, Lukas (author)
-
Nguyen, Hoai Viet (author)
-
Richtera, Lukas (author)
-
show more...
-
Krizkova, Sona (author)
-
Guran, Roman (author)
-
Masarik, Michal (author)
-
Hynek, David (author)
-
Heger, Zbynek (author)
-
Lundberg, Karin (author)
-
- Erikson, Kristofer (author)
- Uppsala universitet,Fasta tillståndets fysik
-
Adam, Vojtech (author)
-
Kizek, Rene (author)
-
show less...
-
(creator_code:org_t)
- 2015-07-20
- 2015
- English.
-
In: Electrophoresis. - : Wiley. - 0173-0835 .- 1522-2683. ; 36:16, s. 1894-1904
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- A novel microfluidic label-free bead-based metallothionein immunosensors was designed. To the surface of superparamagnetic agarose beads coated with protein A, polyclonal chicken IgY specifically recognizing metallothionein (MT) were immobilized via rabbit IgG. The Brdicka reaction was used for metallothionein detection in a microfluidic printed 3D chip. The assembled chip consisted of a single copper wire coated with a thin layer of amalgam as working electrode. Optimization of MT detection using designed microfluidic chip was performed in stationary system as well as in the flow arrangement at various flow rates (0-1800 L/min). In stationary arrangement it is possible to detect MT concentrations up to 30ng/mL level, flow arrangement allows reliable detection of even lower concentration (12.5ng/mL). The assembled miniature flow chip was subsequently tested for the detection of MT elevated levels (at approx. level 100 g/mL) in samples of patients with cancer. The stability of constructed device for metallothionein detection in flow arrangement was found to be several days without any maintenance needed.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Keyword
- Immunosensor
- Label-free
- Metallothionein
- Superparamagnetic particles
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Nejdl, Lukas
-
Nguyen, Hoai Vie ...
-
Richtera, Lukas
-
Krizkova, Sona
-
Guran, Roman
-
Masarik, Michal
-
show more...
-
Hynek, David
-
Heger, Zbynek
-
Lundberg, Karin
-
Erikson, Kristof ...
-
Adam, Vojtech
-
Kizek, Rene
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Chemical Science ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Medical Biotechn ...
-
and Medical Biotechn ...
- Articles in the publication
-
Electrophoresis
- By the university
-
Uppsala University