Cardiac Arrest Alters Regional Ubiquitin Levels in Association with the Blood-Brain Barrier Breakdown and Neuronal Damages in the Porcine Brain

The possibility that ubiquitin expression is altered in cardiac arrest-associated neuropathology was examined in a porcine model using immunohistochemical and biochemical methods. Our observations show that cardiac arrest induces progressive increase in ubiquitin expression in the cortex and hippocampus in a selective and specific manner as compared to corresponding control brains using enzyme-linked immunoassay technique (enzyme-linked immunosorbent assay (ELISA)). Furthermore, immunohistochemical studies showed ubiquitin expression in the neurons exhibiting immunoreaction in the cytoplasm and karyoplasm of distorted or damaged cells. Separate Nissl and ubiquitin staining showed damaged and distorted neurons and in the same cortical region ubiquitin expression indicating that ubiquitin expression after cardiac arrest represents dying neurons. The finding that methylene blue treatment markedly induced neuroprotection following identical cardiac arrest and reduced ubiquitin expression strengthens this view. Taken together, our observations are the first to show that cardiac arrest enhanced ubiquitin expression in the brain that is related to the magnitude of neuronal injury and the finding that methylene blue reduced ubiquitin expression points to its role in cell damage, not reported earlier.

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Anestesi och intensivvård hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Anesthesiology and Intensive Care hsv//eng
Cardiac arrest
Ubiquitin expression
Brain pathology
Neuroprotection
Methylene blue
Transmission electron microscopy
Immunohistochemistry
ELISA

Patnaik, RanjanaUppsala universitet,Anestesiologi och intensivvård (author)
Sharma, ArunaUppsala universitet,Anestesiologi och intensivvård(Swepub:uu)arush975 (author)
Vicente Lafuente, JoseUniv Basque Country, Dept Neurosci, Bilbao, Spain. (author)
Miclescu, AdrianaUppsala universitet,Anestesiologi och intensivvård,Uppsala Univ, Dept Surg Sci Anesthesiol & Intens Care Med, Lab Cerebrovasc Res, S-75185 Uppsala, Sweden.(Swepub:uu)admic838 (author)
Wiklund, LarsUppsala universitet,Anestesiologi och intensivvård(Swepub:uu)larswikl (author)
Uppsala universitetAnestesiologi och intensivvård (creator_code:org_t)

In:Molecular Neurobiology: Springer Science and Business Media LLC52:2, s. 1043-10530893-76481559-1182

By the author/editor: Sharma, Hari Sha ...; Patnaik, Ranjana; Sharma, Aruna; Vicente Lafuente ...; Miclescu, Adrian ...; Wiklund, Lars

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Anesthesiology a ...

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