Genome-wide transcriptional and physiological responses to drought stress in leaves and roots of two willow genotypes

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Pucholt, PascalSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology,Swedish Univ Agr Sci, Dept Plant Biol, Uppsala BioCtr, Linnean Ctr Plant Biol, SE-75007 Uppsala, Sweden. (författare)

Genome-wide transcriptional and physiological responses to drought stress in leaves and roots of two willow genotypes

Artikel/kapitelEngelska2015

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2015-10-12
Springer Science and Business Media LLC,2015
electronicrdacarrier

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LIBRIS-ID:oai:DiVA.org:uu-265815
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-265815URI
https://doi.org/10.1186/s12870-015-0630-2DOI
https://res.slu.se/id/publ/69071URI

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Språk:engelska
Sammanfattning på:engelska

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Correction in BMC Plant Biology (vol 15, 244, 2015) doi: 10.1186/s12870-015-0665-4.
Background: Drought is a major environmental stress that can have severe impacts on plant productivity and survival. Understanding molecular mechanisms of drought responses is crucial in order to breed for drought adapted plant cultivars. The aim of the present study was to investigate phenotypic and transcriptional drought responses in two willow genotypes (520 and 592) originating from an experimental cross between S. viminalis x (S. viminalis x S. schwerinii). Willows are woody perennials in the Salicaceae plant family that are grown as bioenergy crops worldwide. Methods: An experiment was conducted where plants were exposed to drought and different eco-physiological parameters were assessed. RNA-seq data was furthermore generated with the Illumina technology from root tips and leaves from plants grown in drought and well-watered (WW) conditions. The RNA-seq data was assembled de novo with the Trinity assembler to create a reference gene set to which the reads were mapped in order to obtain differentially expressed genes (DEGs) between the drought and WW conditions. To investigate molecular mechanisms involved in the drought response, GO enrichment analyses were conducted. Candidate genes with a putative function in the drought response were also identified. Results: A total of 52,599 gene models were obtained and after filtering on gene expression (FPKM >= 1), 35,733 gene models remained, of which 24,421 contained open reading frames. A total of 5,112 unique DEGs were identified between drought and WW conditions, of which the majority were found in the root tips. Phenotypically, genotype 592 displayed less growth reduction in response to drought compared to genotype 520. At the transcriptional level, genotype 520 displayed a greater response in the leaves as more DEGs were found in genotype 520 compared to genotype 592. In contrast, the transcriptional responses in the root tips were rather similar between the two genotypes. A core set of candidate genes encoding proteins with a putative function in drought response was identified, for example MYBs and bZIPs as well as chlorophyll a/b binding proteins. Discussion: We found substantial differences in drought responses between the genotypes, both at the phenotypic and transcriptional levels. In addition to the genotypic variation in several traits, we also found indications for genotypic variation in trait plasticity, which could play a role in drought adaptation. Furthermore, the two genotypes displayed overall similar transcriptional responses in the root tips, but more variation in the leaves. It is thus possible that the observed phenotypic differences could be a result of transcriptional differences mostly at the leaf level. Conclusions: This study has contributed to a better general understanding of drought responses in woody plants, specifically in willows, and has implications for breeding research towards more drought adapted plants.

Ämnesord och genrebeteckningar

NATURVETENSKAP Biologi Genetik hsv//swe
NATURAL SCIENCES Biological Sciences Genetics hsv//eng
LANTBRUKSVETENSKAPER Lantbruksvetenskap, skogsbruk och fiske Skogsvetenskap hsv//swe
AGRICULTURAL SCIENCES Agriculture, Forestry and Fisheries Forest Science hsv//eng
LANTBRUKSVETENSKAPER Lantbruksvetenskap, skogsbruk och fiske Jordbruksvetenskap hsv//swe
AGRICULTURAL SCIENCES Agriculture, Forestry and Fisheries Agricultural Science hsv//eng
Drought stress
Response to drought
RNA-seq
De novo assembly
Salix
Differentially expressed genes
Candidate genes

Biuppslag (personer, institutioner, konferenser, titlar ...)

Sjödin, PerSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Uppsala universitet,Evolutionsbiologi,Swedish Univ Agr Sci, Dept Plant Biol, Uppsala BioCtr, Linnean Ctr Plant Biol, SE-75007 Uppsala, Sweden.,Institutionen för växtbiologi,Department of Plant Biology,Uppsala University(Swepub:slu)47497 (författare)
Weih, MartinSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtproduktionsekologi,Department of Crop Production Ecology,Swedish Univ Agr Sci, Dept Crop Prod Ecol, Linnean Ctr Plant Biol, SE-75007 Uppsala, Sweden.(Swepub:slu)49551 (författare)
Rönnberg Wästljung, Ann-ChristinSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology,Swedish Univ Agr Sci, Dept Plant Biol, Uppsala BioCtr, Linnean Ctr Plant Biol, SE-75007 Uppsala, Sweden.(Swepub:slu)48124 (författare)
Berlin Kolm, SofiaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology,Swedish Univ Agr Sci, Dept Plant Biol, Uppsala BioCtr, Linnean Ctr Plant Biol, SE-75007 Uppsala, Sweden.(Swepub:slu)48551 (författare)
Sveriges lantbruksuniversitetInstitutionen för växtbiologi (creator_code:org_t)
Sveriges lantbruksuniversitet

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Ingår i:BMC Plant Biology: Springer Science and Business Media LLC151471-2229

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Av författaren/redakt...: Pucholt, Pascal; Sjödin, Per; Weih, Martin; Rönnberg Wästlju ...; Berlin Kolm, Sof ...

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