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Biobanking multifoc...
Biobanking multifocal breast carcinomas : sample adequacy with regard to histology and DNA content
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- Pekar, Gyula (författare)
- Department of Pathology and Clinical Cytology, Central Hospital Falun, Falun, Sweden
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- Davies, Hanna (författare)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
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- Lukacs, Agnes P (författare)
- Department of Pathology and Clinical Cytology, Central Hospital Falun, Falun, Sweden
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- Forsberg, Lars (författare)
- Uppsala universitet,Medicinsk genetik och genomik
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- Hellberg, Dan, 1953- (författare)
- Uppsala universitet,Centrum för klinisk forskning Dalarna,Institutionen för kvinnors och barns hälsa,Reproduktiv hälsa/Sundström Poromaa
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- Dumanski, Jan (författare)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
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- Tot, Tibor (författare)
- Department of Pathology and Clinical Cytology, Central Hospital Falun, Falun, Sweden
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(creator_code:org_t)
- 2015-08-12
- 2016
- Engelska.
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Ingår i: Histopathology. - : Wiley. - 0309-0167 .- 1365-2559. ; 68:3, s. 411-421
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- AIMS: To determine the volume of tumoral and normal breast tissue containing sufficient DNA (>2 μg/sample) for genetic platforms and biobanking, with a focus on multifocality, tumoral heterogeneity, and factors that critically influence sample acceptability.METHODS AND RESULTS: We examined 57 breast surgical specimens with multifocal (46/57) and unifocal (11/57) cancers. Punch biopsies were obtained from tissue slices under multimodal radiological guidance, and the colour-coded sampling sites were identified in large-format histology slides. The study comprised 415 DNA isolations from tumour (n = 105) and normal (n = 283) tissue, including skin (n = 27) samples. A single 2-mm core from invasive tumour contained sufficient DNA in 91.4% (96/105) of cases, depending on tumour type (3.8-108.2 μg/sample), number and size of additional foci in multifocal cases (P = 0.001), tumour consistency, and degree of necrosis. Three biopsies obtained with a 4-mm device were required from normal breast tissue, at least 10 mm from the tumour. Cold ischaemia for up to 82 min did not influence the yield of DNA.CONCLUSIONS: Radiological disease mapping is useful for guiding optimal specimen slicing and for targeting breast lesions. A single 2-mm core from tumour and multiple 4-mm cores from normal breast tissue yield adequate DNA in the majority of samples.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- biobanking; breast cancer; DNA quality and quantity; heterogeneity; multifocality
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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