Comprehensive Proteomic Study of the Antiproliferative Activity of a Polyphenol-Enriched Rosemary Extract on Colon Cancer Cells Using Nanoliquid Chromatography-Orbitrap MS/MS

Valdes, AlbertoCSIC, Inst Food Sci Res CIAL, Lab Food, Calle Nicolas Cabrera 9, Madrid 28049, Spain. (author)

In this work, a proteomics strategy based on nanoliquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) using an Orbitrap high-resolution mass spectrometer together with stable isotope dimethyl labeling (DML) is applied to quantitatively examine relative changes in the protein fraction of HT-29 human colon cancer cells treated with different concentrations of a polyphenol-enriched rosemary extract over the time. The major objective of this study was to gain insights into the antiproliferative mechanisms induced by rosemary polyphenols. Using this methodology, 1909 and 698 proteins were identified and quantified in cell extracts. The polyphenol-enriched rosemary extract treatment changed the expression of several proteins in a time- and concentration-dependent manner. Most of the altered proteins are implicated, in the activation of Nrf2 transcription factor and the unfolded protein response. In conclusion, rosemary polyphenols induced proteomic changes that were related to the attenuation of aggresome formation and activation of autophagy to alleviate cellular stress..

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
antiproliferative activity
foodomics
colon cancer
dimethyl labeling
HT-29 cells
mass spectrometry
quantitative proteomics
rosemary extract

Artemenko, Konstantin A.Uppsala universitet,Analytisk kemi(Swepub:uu)konar476 (author)
Bergquist, JonasUppsala universitet,Analytisk kemi(Swepub:uu)jbe25356 (author)
Garcia-Canas, VirginiaCSIC, Inst Food Sci Res CIAL, Lab Food, Calle Nicolas Cabrera 9, Madrid 28049, Spain. (author)
Cifuentes, AlejandroCSIC, Inst Food Sci Res CIAL, Lab Food, Calle Nicolas Cabrera 9, Madrid 28049, Spain. (author)
CSIC, Inst Food Sci Res CIAL, Lab Food, Calle Nicolas Cabrera 9, Madrid 28049, Spain.Analytisk kemi (creator_code:org_t)

In:Journal of Proteome Research: American Chemical Society (ACS)15:6, s. 1971-19851535-38931535-3907

By the author/editor: Valdes, Alberto; Artemenko, Konst ...; Bergquist, Jonas; Garcia-Canas, Vi ...; Cifuentes, Aleja ...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

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