Endometrial transcriptome analysis indicates superiority of natural over artificial cycles in recurrent implantation failure patients undergoing frozen embryo transfer

Altmae, SigneCompetence Ctr Hlth Technol, Tartu, Estonia.;Univ Granada, Sch Med, Dept Paediat, Granada, Spain. (author)

Little consensus has been reached on the best protocol for endometrial preparation for frozen embryo transfer (FET). It is not known how, and to what extent, hormone supplementation in artificial cycles influences endometrial preparation for embryo implantation at a molecular level, especially in patients who have experienced recurrent implantation failure. Transcriptome analysis of 15 endometrial biopsy samples at the time of embryo implantation was used to compare two different endometrial preparation protocols, natural versus artificial cycles, for FET in women who have experienced recurrent implantation failure compared with fertile women. IPA and DAVID were used for functional analyses of differentially expressed genes. The TRANSFAC database was used to identify oestrogen and progesterone response elements upstream of differentially expressed genes. Cluster analysis demonstrated that natural cycles are associated with a better endometrial receptivity transcriptome than artificial cycles. Artificial cycles seemed to have a stronger negative effect on expression of genes and pathways crucial for endometrial receptivity, including ESR2, FSHR, LEP, and several interleukins and matrix metalloproteinases. Significant overrepresentation of oestrogen response elements among the genes with deteriorated expression in artificial cycles (P < 0.001) was found; progesterone response elements predominated in genes with amended expression with artificial cycles (P = 0.0052).

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Reproduktionsmedicin och gynekologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Obstetrics, Gynaecology and Reproductive Medicine hsv//eng
artificial cycle
endometrial receptivity
frozen embryo transfer
hormone response elements
recurrent implantation failure
unexplained female infertility

Tamm-Rosenstein, KarinTallinn Univ Technol, Dept Gene Technol, EE-19086 Tallinn, Estonia. (author)
Esteban, Francisco J.Univ Jaen, Dept Expt Biol, Jaen, Spain. (author)
Simm, JaakTallinn Univ Technol, Dept Gene Technol, EE-19086 Tallinn, Estonia. (author)
Kolberg, LiisUniv Tartu, Inst Comp Sci, Ulikooli 18, EE-50090 Tartu, Estonia. (author)
Peterson, HediUniv Tartu, Inst Comp Sci, Ulikooli 18, EE-50090 Tartu, Estonia.;Quretec Ltd, Tartu, Estonia. (author)
Metsis, MadisCompetence Ctr Hlth Technol, Tartu, Estonia.;Tallinn Univ, Sch Nat Sci & Hlth, EE-10120 Tallinn, Estonia. (author)
Haldre, KaiWest Tallinn Cent Hosp Womens Clin, Ctr Reprod Med, Tallinn, Estonia. (author)
Horcajadas, Jose A.Hosp Miguel Servet, Araid I CS, Zaragoza, Spain. (author)
Salumets, AndresCompetence Ctr Hlth Technol, Tartu, Estonia.;Univ Tartu, Dept Obstet & Gynaecol, Ulikooli 18, EE-50090 Tartu, Estonia. (author)
Stavreus-Evers, AnneliUppsala universitet,Obstetrik & gynekologi(Swepub:uu)annst384 (author)
Competence Ctr Hlth Technol, Tartu, Estonia.;Univ Granada, Sch Med, Dept Paediat, Granada, Spain.Tallinn Univ Technol, Dept Gene Technol, EE-19086 Tallinn, Estonia. (creator_code:org_t)

In:Reproductive BioMedicine Online: Elsevier BV32:6, s. 597-6131472-64831472-6491

By the author/editor: Altmae, Signe; Tamm-Rosenstein, ...; Esteban, Francis ...; Simm, Jaak; Kolberg, Liis; Peterson, Hedi; show more...; Metsis, Madis; Haldre, Kai; Horcajadas, Jose ...; Salumets, Andres; Stavreus-Evers, ...; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Obstetrics Gynae ...

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