Uptake, delivery, and anticancer activity of thymoquinone nanoparticles in breast cancer cells

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Sökning: L773:1388 0764 OR L773:1572 896X > Uptake, delivery, a...

Fakhoury, IsabelleAmer Univ Beirut, Dept Biol, Beirut, Lebanon. (författare)

Uptake, delivery, and anticancer activity of thymoquinone nanoparticles in breast cancer cells

Artikel/kapitelEngelska2016

Förlag, utgivningsår, omfång ...

2016-07-25
Springer Science and Business Media LLC,2016
printrdacarrier

Nummerbeteckningar

LIBRIS-ID:oai:DiVA.org:uu-303298
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-303298URI
https://doi.org/10.1007/s11051-016-3517-8DOI

Kompletterande språkuppgifter

Språk:engelska
Sammanfattning på:engelska

Ingår i deldatabas

SwePubSwePub

Klassifikation

Ämneskategori:ref swepub-contenttype
Ämneskategori:art swepub-publicationtype

Anmärkningar

Thymoquinone (TQ) is a promising anticancer molecule but its development is hindered by its limited bioavailability. Drug encapsulation is commonly used to overcome low drug solubility, limited bioavailability, and nonspecific targeting. In this project, TQ nanoparticles (TQ-NP) were synthesized and characterized. The cytotoxicity of the NP was investigated in nontumorigenic MCF-10-A breast cells, while the uptake, distribution, as well as the anticancer potential were investigated in MCF-7 and MDA-MB-231 breast cancer cells. Flash Nanoprecipitation and dynamic light scattering coupled with scanning electron microscopy were used to prepare and characterize TQ-NP prior to measuring their anticancer potential by MTT assay. The uptake and subcellular intake of TQ-NP were evaluated by fluorometry and confocal microscopy. TQ-NP were stable with a hydrodynamic average diameter size around 100 nm. Entrapment efficiency and loading content of TQ-NP were high (around 80 and 50 %, respectively). In vitro, TQ-NP had equal or enhanced anticancer activity effects compared to TQ in MCF-7 and aggressive MDA-MB-231 breast cancer cells, respectively, with no significant cytotoxicity of the blank NP. In addition, TQ and TQ-NP were relatively nontoxic to MCF-10-A normal breast cells. TQ-NP uptake mechanism was both time and concentration dependent. Treatment with inhibitors of endocytosis suggested the involvement of caveolin in TQ-NP uptake. This was further confirmed by subcellular localization findings showing the colocalization of TQ-NP with caveolin and transferrin as well as with the early and late markers of endocytosis. Altogether, the results describe an approach for the enhancement of TQ anticancer activity and uncover the mechanisms behind cell-TQ-NP interaction.

Ämnesord och genrebeteckningar

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Cancer och onkologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Cancer and Oncology hsv//eng
Thymoquinone
Breast cancer
Nanoparticles
Uptake
Endocytosis

Biuppslag (personer, institutioner, konferenser, titlar ...)

Saad, WalidAmer Univ Beirut, Dept Chem & Petr Engn, Beirut, Lebanon. (författare)
Bouhadir, KamalAmer Univ Beirut, Dept Chem, Beirut, Lebanon. (författare)
Nygren, PeterUppsala universitet,Experimentell och klinisk onkologi(Swepub:uu)peterng (författare)
Schneider-Stock, RegineUniv Erlangen Nurnberg, Inst Pathol, Expt Tumor Pathol, Erlangen, Germany. (författare)
Gali-Muhtasib, HalaAmer Univ Beirut, Dept Biol, Beirut, Lebanon.;Amer Univ Beirut, Dept Anat, Cell Biol, Physiol,Fac Med, Beirut, Lebanon. (författare)
Amer Univ Beirut, Dept Biol, Beirut, Lebanon.Amer Univ Beirut, Dept Chem & Petr Engn, Beirut, Lebanon. (creator_code:org_t)

Sammanhörande titlar

Ingår i:Journal of nanoparticle research: Springer Science and Business Media LLC18:71388-07641572-896X

Internetlänk

Hitta via bibliotek

Journal of nanoparticle research (Sök värdpublikationen i LIBRIS)

Till lärosätets databas

Hitta mer i SwePub

Av författaren/redakt...: Fakhoury, Isabel ...; Saad, Walid; Bouhadir, Kamal; Nygren, Peter; Schneider-Stock, ...; Gali-Muhtasib, H ...

Om ämnet

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Cancer och onkol ...

Artiklar i publikationen: Journal of nanop ...

Av lärosätet: Uppsala universitet

Sök utanför SwePub

Sök vidare i:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se