The infectious particle of insect-borne totivirus-like Omono River virus has raised ridges and lacks fibre complexes

Omono River virus (OmRV) is a double-stranded RNA virus isolated from Culex mosquitos, and it belongs to a group of unassigned insect viruses that appear to be related to Totiviridae. This paper describes electron cryo-microscopy (cryoEM) structures for the intact OmRV virion to 8.9 angstrom resolution and the structure of the empty virus-like-particle, that lacks RNA, to 8.3 angstrom resolution. The icosahedral capsid contains 120-subunits and resembles another closely related arthropod-borne totivirus-like virus, the infectious myonecrosis virus (IMNV) from shrimps. Both viruses have an elevated plateau around their icosahedral 5-fold axes, surrounded by a deep canyon. Sequence and structural analysis suggests that this plateau region is mainly composed of the extended C-terminal region of the capsid proteins. In contrast to IMNV, the infectious form of OmRV lacks extensive fibre complexes at its 5-fold axes as directly confirmed by a contrast-enhancement technique, using Zernike phase-contrast cryo-EM. Instead, these fibre complexes are replaced by a short "plug" structure at the five-fold axes of OmRV. OmRV and IMNV have acquired an extracellular phase, and the structures at the five-fold axes may be significant in adaptation to cell-to-cell transmission in metazoan hosts.

Miyazaki, NaoyukiNatl Inst Physiol Sci, Okazaki, Aichi, Japan. (författare)
Larsson, Daniel S. D.Uppsala universitet,Molekylär biofysik(Swepub:uu)dalar193 (författare)
Kobayashi, DaisukeNatl Inst Infect Dis, Dept Med Entomol, Tokyo, Japan. (författare)
Svenda, MartinUppsala universitet,Molekylär biofysik(Swepub:uu)msv31659 (författare)
Mühlig, KerstinUppsala universitet,Molekylär biofysik(Swepub:uu)kermu539 (författare)
Maia, Filipe R. N. C.Uppsala universitet,Molekylär biofysik(Swepub:uu)fimai103 (författare)
Gunn, Laura H.Uppsala universitet,Molekylär biofysik(Swepub:uu)laugu236 (författare)
Isawa, HaruhikoNatl Inst Infect Dis, Dept Med Entomol, Tokyo, Japan. (författare)
Kobayashi, MutsuoNatl Inst Infect Dis, Dept Med Entomol, Tokyo, Japan. (författare)
Sawabe, KyokoNatl Inst Infect Dis, Dept Med Entomol, Tokyo, Japan. (författare)
Murata, KazuyoshiNatl Inst Physiol Sci, Okazaki, Aichi, Japan. (författare)
Hajdu, JanosUppsala universitet,Molekylär biofysik(Swepub:uu)janohajd (författare)
Uppsala universitetMolekylär biofysik (creator_code:org_t)

Av författaren/redakt...: Okamoto, Kenta; Miyazaki, Naoyuk ...; Larsson, Daniel ...; Kobayashi, Daisu ...; Svenda, Martin; Mühlig, Kerstin; visa fler...; Maia, Filipe R. ...; Gunn, Laura H.; Isawa, Haruhiko; Kobayashi, Mutsu ...; Sawabe, Kyoko; Murata, Kazuyosh ...; Hajdu, Janos; visa färre...

Om ämnet

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.