SwePub
Sök i LIBRIS databas

  Extended search

id:"swepub:oai:DiVA.org:uu-306609"
 

Search: id:"swepub:oai:DiVA.org:uu-306609" > Coherent Diffractiv...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist
  • Hantke, Max Felix,1984-Uppsala universitet,Molekylär biofysik (author)

Coherent Diffractive Imaging with X-ray Lasers

  • BookEnglish2016

Publisher, publication year, extent ...

  • Uppsala :Acta Universitatis Upsaliensis,2016
  • 84 s.
  • electronicrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-306609
  • ISBN:9789155497484
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-306609URI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:vet swepub-contenttype
  • Subject category:dok swepub-publicationtype

Series

  • Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology,1651-6214 ;1451

Notes

  • The newly emerging technology of X-ray free-electron lasers (XFELs) has the potential to revolutionise molecular imaging. XFELs generate very intense X-ray pulses and predictions suggest that they may be used for structure determination to atomic resolution even for single molecules. XFELs produce femtosecond pulses that outrun processes of radiation damage and permit the study of structures at room temperature and of structural dynamics.While the first demonstrations of flash X-ray diffractive imaging (FXI) on biological particles were encouraging, they also revealed technical challenges. In this work we demonstrated how some of these challenges can be overcome. We exemplified, with heterogeneous cell organelles, how tens of thousands of FXI diffraction patterns can be collected, sorted, and analysed in an automatic data processing pipeline. We improved  image resolution and reduced problems with missing data. We validated, described, and deposited the experimental data in the Coherent X-ray Imaging Data Bank.We demonstrated that aerosol injection can be used to collect FXI data at high hit ratios and with low background. We reduced problems with non-volatile sample contaminants by decreasing aerosol droplet sizes from ~1000 nm to ~150 nm. We achieved this by adapting an electrospray aerosoliser to the Uppsala sample injector. Mie scattering imaging was used as a diagnostic tool to measure positions, sizes, and velocities of individual injected particles.XFEL experiments generate large amounts of data at high rates. Preparation, execution, and data analysis of these experiments benefits from specialised software. In this work we present new open-source software tools that facilitates prediction, online-monitoring, display, and pre-processing of XFEL diffraction data.We hope that this work is a valuable contribution in the quest of transitioning FXI from its first experimental demonstration into a technique that fulfills its potentials.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Maia, Filipe R. N. C.,Dr.Uppsala universitet,Molekylär biofysik (thesis advisor)
  • Hajdu, Janos,Prof.Uppsala universitet,Molekylär biofysik (thesis advisor)
  • Svenda, Martin,Dr.Uppsala universitet,Molekylär biofysik (thesis advisor)
  • Weckert, Edgar,Prof.Hamburg University (opponent)
  • Uppsala universitetMolekylär biofysik (creator_code:org_t)

Internet link

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Search outside SwePub

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view