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Resistin Gene Expre...
Resistin Gene Expression is Downregulated in CD4(+) T Helper Lymphocytes and CD14(+) Monocytes in Rheumatoid Arthritis Responding to TNF-alpha Inhibition
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- Nagaev, I. (författare)
- Norrlands Univ Hosp, Dept Clin Microbiol, Div Clin Immunol, Umea, Sweden.
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- Andersen, M. (författare)
- Aalborg Univ, Dept Hlth Sci & Technolgy, North Denmark Reg Hosp, Dept Med, Aalborg, Denmark.
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- Olesen, M. K. (författare)
- Aalborg Univ, Dept Hlth Sci & Technolgy, North Denmark Reg Hosp, Dept Med, Aalborg, Denmark.
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- Nagaeva, O. (författare)
- Norrlands Univ Hosp, Dept Clin Microbiol, Div Clin Immunol, Umea, Sweden.
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- Wikberg, Jarl (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Mincheva-Nilsson, L. (författare)
- Norrlands Univ Hosp, Dept Clin Microbiol, Div Clin Immunol, Umea, Sweden.
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- Andersen, G. N. (författare)
- Aalborg Univ, Dept Clin Med, North Denmark Reg Hosp, Dept Rheumatol, Aalborg, Denmark.
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Norrlands Univ Hosp, Dept Clin Microbiol, Div Clin Immunol, Umea, Sweden Aalborg Univ, Dept Hlth Sci & Technolgy, North Denmark Reg Hosp, Dept Med, Aalborg, Denmark. (creator_code:org_t)
- 2016-09-26
- 2016
- Engelska.
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Ingår i: Scandinavian Journal of Immunology. - : Wiley. - 0300-9475 .- 1365-3083. ; 84:4, s. 229-236
- Relaterad länk:
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https://onlinelibrar...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Rheumatoid arthritis (RA) is caused by complex interactions between immune cells and sustained by Th1 response cytokines. Resistin [resistance to insulin; (RETN)] is an inflammatory cytokine, first discovered in murine adipocytes. In man, RETN is mainly secreted by monocytes. The distinct role of RETN in the immune reaction is uncertain; however, RETN has pro-inflammatory, profibrotic and possibly tolerogenic properties. The aim was to assess the reaction of RETN gene expression to TNF-alpha inhibition (I) in pathogenetic immune cell subsets in RA, in the context of Th1, inflammatory and regulatory cytokine gene expressions. Accordingly, we measured RETN, IFN-gamma, TNF-beta, IL-1 beta, TNF-alpha, TGF-beta and IL-10 gene expressions in CD14(+) monocytes, CD4(+) T helper (Th) lymphocytes (ly), CD8(+) T cytotoxic (Tc) ly and CD19(+) B ly in active RA before and 3 months after start of TNF-alpha I. Leucocyte subsets were separated by specific monoclonal antibody-covered beads, RNA extracted and levels of RETN, Th1 response, inflammatory and regulatory cytokine mRNAs measured by quantitative reverse transcription-polymerase chain reaction technique. We found that TNF-alpha I caused a significant downregulation of RETN gene expression in CD14(+) monocytes and CD4(+) Th ly and was unchanged in CD8(+) Tc ly and CD19(+) B ly. Both in active RA and during TNF-alpha I, RETN mRNA levels were significantly higher in CD14(+) monocytes than in all other examined cell types. In monocytes, fold change in RETN and TGF-beta gene expressions upon TNF-alpha I correlated significantly. Our findings indicate that RETN has pro-inflammatory as well as proresolving roles in active RA.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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