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Integrating mass sp...
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Landreh, Michael
(author)
Integrating mass spectrometry with MD simulations reveals the role of lipids in Na+/H+ antiporters
- Article/chapterEnglish2017
Publisher, publication year, extent ...
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2017-01-10
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Springer Science and Business Media LLC,2017
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electronicrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-314649
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-314649URI
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https://doi.org/10.1038/ncomms13993DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-139365URI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Authors Marklund and Uzdavinys contributed equally to this work.
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Na+/H+ antiporters are found in all kingdoms of life and exhibit catalysis rates that are among the fastest of all known secondary-active transporters. Here we combine ion mobility mass spectrometry and molecular dynamics simulations to study the conformational stability and lipid-binding properties of the Na+/H+ exchanger NapA from Thermus thermophilus and compare this to the prototypical antiporter NhaA from Escherichia coli and the human homologue NHA2. We find that NapA and NHA2, but not NhaA, form stable dimers and do not selectively retain membrane lipids. By comparing wild-type NapA with engineered variants, we show that the unfolding of the protein in the gas phase involves the disruption of inter-domain contacts. Lipids around the domain interface protect the native fold in the gas phase by mediating contacts between the mobile protein segments. We speculate that elevator-type antiporters such as NapA, and likely NHA2, use a subset of annular lipids as structural support to facilitate large-scale conformational changes within the membrane.
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Marklund, Erik GUppsala universitet,Biokemi,Erik Marklund(Swepub:uu)ermar499
(author)
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Uzdavinys, PovilasStockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)pouz5783
(author)
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Degiacomi, Matteo T.
(author)
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Coincon, MathieuStockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)mcoin
(author)
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Gault, Joseph
(author)
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Gupta, Kallol
(author)
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Liko, Idlir
(author)
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Benesch, Justin L. P.
(author)
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Drew, DavidStockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)drew
(author)
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Robinson, Carol V.
(author)
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Uppsala universitetBiokemi
(creator_code:org_t)
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In:Nature Communications: Springer Science and Business Media LLC82041-1723
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Landreh, Michael
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Marklund, Erik G
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Uzdavinys, Povil ...
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Degiacomi, Matte ...
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Coincon, Mathieu
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Gault, Joseph
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Gupta, Kallol
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Liko, Idlir
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Benesch, Justin ...
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Drew, David
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Robinson, Carol ...
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Stockholm University