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Design and validati...
Design and validation of an STR hexaplex assay for DNA profiling of grapevine cultivars
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- Drabek, Jiri (författare)
- Palacky Univ, Fac Med & Dent, Inst Mol & Translat Med, Hnevotinska 5, Olomouc 77900, Czech Republic.
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- Smolikova, Michaela (författare)
- Palacky Univ, Dept Biochem, Olomouc, Czech Republic.
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- Kalendar, Ruslan (författare)
- Univ Helsinki, LUKE BI Plant Genome Dynam, Inst Biotechnol, Helsinki, Finland.;Minist Educ & Sci Republ Kazakhstan, Sci Comm, RSE Natl Ctr Biotechnol, Astana, Kazakhstan.
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- Lopes Pinto, Fernando A. (författare)
- Uppsala universitet,Institutionen för fotokemi och molekylärvetenskap
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- Pavlousek, Pavel (författare)
- Mendel Univ Brno, Dept Viticulture & Enol, Brno, Czech Republic.
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- KleparnIk, Karel (författare)
- ASCR, Inst Analyt Chem, Brno, Czech Republic.
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- Frebort, Ivo (författare)
- Palacky Univ, Fac Sci, Ctr Reg Hana Biotechnol & Agr Res, Olomouc, Czech Republic.
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Palacky Univ, Fac Med & Dent, Inst Mol & Translat Med, Hnevotinska 5, Olomouc 77900, Czech Republic Palacky Univ, Dept Biochem, Olomouc, Czech Republic. (creator_code:org_t)
- 2016-10-26
- 2016
- Engelska.
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Ingår i: Electrophoresis. - : Wiley. - 0173-0835 .- 1522-2683. ; 37:23-24, s. 3059-3067
- Relaterad länk:
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https://helda.helsin...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Although the analysis of length polymorphism at STR loci has become a method of choice for grape cultivar identification, the standardization of methods for this purpose lags behind that of methods for DNA profiling in human and animal forensic genetics. The aim of this study was thus to design and validate a grapevine STR protocol with a practically useful level of multiplexing. Using free bioinformatics tools, published primer sequences, and nucleotide databases, we constructed and optimized a primer set for the simultaneous analysis of six STR loci (VVIi51, scu08vv, scu05vv, VVMD17, VrZAG47, and VrZAG83) by multiplex PCR and CE with laser-induced fluorescence, and tested it on 90 grape cultivars. The new protocol requires subnanogram quantities of the DNA template and enables automated, high-throughput genetic analysis with reasonable discriminatory power. As such, it represents a step toward further standardization of grape DNA profiling.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Grapevine DNA analysis
- Multiplex PCR
- STRs
- Vitis vinifera L
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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