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Large Is Fast, Smal...
Large Is Fast, Small Is Tight : Determinants of Speed and Affinity in Subunit Capture by a Periplasmic Chaperone
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- Di Yu, Xiao (författare)
- Swedish Univ Agr Sci, Dept Mol Biol, Uppsala Bioctr, BMC, SE-75324 Uppsala, Sweden.
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- Fooks, Laura J. (författare)
- Univ Reading, Sch Biol Sci, Reading RG6 6AJ, Berks, England.
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- Moslehi-Mohebi, Elham (författare)
- Univ Reading, Sch Biol Sci, Reading RG6 6AJ, Berks, England.
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- Tischenko, Vladimir M. (författare)
- Inst Biol Instrumentat, Pushchino 142292, Russia.
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- Askarieh, Glareh (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för molekylärbiologi,Department of Molecular Biology
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- Knight, Stefan D. (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Uppsala universitet,Struktur- och molekylärbiologi,Swedish Univ Agr Sci, Dept Mol Biol, Uppsala Bioctr, BMC, SE-75324 Uppsala, Sweden.,Institutionen för molekylärbiologi,Department of Molecular Biology
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- MacIntyre, Sheila (författare)
- Univ Reading, Sch Biol Sci, Reading RG6 6AJ, Berks, England.
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- Zavialov, Anton V. (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för molekylärbiologi,Department of Molecular Biology,University of Turku
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- Yu, Xiaodi (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för molekylärbiologi,Department of Molecular Biology
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Swedish Univ Agr Sci, Dept Mol Biol, Uppsala Bioctr, BMC, SE-75324 Uppsala, Sweden Univ Reading, Sch Biol Sci, Reading RG6 6AJ, Berks, England. (creator_code:org_t)
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- Elsevier BV, 2012
- 2012
- Engelska.
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Ingår i: Journal of Molecular Biology. - : Elsevier BV. - 0022-2836 .- 1089-8638. ; 417:4, s. 294-308
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The chaperone/usher pathway assembles surface virulence organelles of Gram-negative bacteria, consisting of fibers of linearly polymerized protein subunits. Fiber subunits are connected through 'donor strand complementation': each subunit completes the immunoglobulin (Ig)-like fold of the neighboring subunit by donating the seventh beta-strand in trans. Whereas the folding of Ig domains is a fast first-order process, folding of Ig modules into the fiber conformation is a slow second-order process. Periplasmic chaperones separate this process in two parts by forming transient complexes with subunits. Interactions between chaperones and subunits are also based on the principle of donor strand complementation. In this study, we have performed mutagenesis of the binding motifs of the Caf1M chaperone and Caf1 capsular subunit from Yersinia pestis and analyzed the effect of the mutations on the structure, stability, and kinetics of Caf1M-Caf1 and Caf1-Caf1 interactions. The results suggest that a large hydrophobic effect combined with extensive main-chain hydrogen bonding enables Caf1M to rapidly bind an early folding intermediate of Caf1 and direct its partial folding. The switch from the Caf1M-Caf1 contact to the less hydrophobic, but considerably tighter and less dynamic Caf1-Caf1 contact occurs via the zip-out-zip-in donor strand exchange pathway with pocket 5 acting as the initiation site. Based on these findings, Caf1M was engineered to bind Caf1 faster, tighter, or both faster and tighter. To our knowledge, this is the first successful attempt to rationally design an assembly chaperone with improved chaperone function.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- assembly
- folding
- secretion
- structure
- protein design
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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