Sökning: WFRF:(Flyvbjerg Henrik) >
Antioxidant treatme...
Antioxidant treatment attenuates lactate production in diabetic nephropathy
-
- Laustsen, Christoffer (författare)
- Aarhus University, Denmark
-
- Nielsen, Per Mose (författare)
- Aarhus University, Denmark
-
- Nørlinger, Thomas Stokholm (författare)
- Aarhus University, Denmark
-
visa fler...
-
- Qi, Haiyun (författare)
- Aarhus University, Denmark
-
- Pedersen, Uffe Kjærgaard (författare)
- Aarhus University, Denmark
-
- Bertelsen, Lotte Bonde (författare)
- Aarhus University, Denmark
-
- Østergaard, Jakob Appel (författare)
- Aarhus University, Denmark; Danish Diabet Acad, Denmark
-
- Flyvbjerg, Allan (författare)
- Aarhus University, Denmark
-
- Ardenkjær-Larsen, Jan Henrik (författare)
- GE Healthcare, Denmark; Technical University of Denmark, Denmark
-
- Palm, Fredrik, 1973- (författare)
- Linköpings universitet,Uppsala universitet,Integrativ Fysiologi,Avdelningen för läkemedelsforskning,Medicinska fakulteten,Uppsala University, Sweden
-
- Stødkilde-Jørgensen, Hans (författare)
- Aarhus University, Denmark
-
visa färre...
-
(creator_code:org_t)
- American Physiological Society, 2017
- 2017
- Engelska.
-
Ingår i: American Journal of Physiology - Renal Physiology. - : American Physiological Society. - 0363-6127 .- 1522-1466 .- 1931-857X. ; 312:1, s. F192-F199
- Relaterad länk:
-
https://www.physiolo...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
visa färre...
Abstract
Ämnesord
Stäng
- The early progression of diabetic nephropathy is notoriously difficult to detect and quantify before the occurrence of substantial histological damage. Recently, hyperpolarized [1-(13)C]pyruvate has demonstrated increased lactate production in the kidney early after the onset of diabetes, implying increased lactate dehydrogenase activity as a consequence of increased nicotinamide adenine dinucleotide substrate availability due to upregulation of the polyol pathway, i.e., pseudohypoxia. In this study, we investigated the role of oxidative stress in mediating these metabolic alterations using state-of-the-art hyperpolarized magnetic resonance (MR) imaging. Ten-week-old female Wistar rats were randomly divided into three groups: healthy controls, untreated diabetic (streptozotocin treatment to induce insulinopenic diabetes), and diabetic, receiving chronic antioxidant treatment with TEMPOL (4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl) via the drinking water. Examinations were performed 2, 3, and 4 wk after the induction of diabetes by using a 3T Clinical MR system equipped with a dual tuned (13)C/(1)H-volume rat coil. The rats received intravenous hyperpolarized [1-(13)C]pyruvate and were imaged using a slice-selective (13)C-IDEAL spiral sequence. Untreated diabetic rats showed increased renal lactate production compared with that shown by the controls. However, chronic TEMPOL treatment significantly attenuated diabetes-induced lactate production. No significant effects of diabetes or TEMPOL were observed on [(13)C]alanine levels, indicating an intact glucose-alanine cycle, or [(13)C]bicarbonate, indicating normal flux through the Krebs cycle. In conclusion, this study demonstrates that diabetes-induced pseudohypoxia, as indicated by an increased lactate-to-pyruvate ratio, is significantly attenuated by antioxidant treatment. This demonstrates a pivotal role of oxidative stress in renal metabolic alterations occurring in early diabetes.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Nyckelord
- MRI
- TEMPOL
- diabetic nephropathy
- hyperpolarization
- renal metabolism
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Laustsen, Christ ...
-
Nielsen, Per Mos ...
-
Nørlinger, Thoma ...
-
Qi, Haiyun
-
Pedersen, Uffe K ...
-
Bertelsen, Lotte ...
-
visa fler...
-
Østergaard, Jako ...
-
Flyvbjerg, Allan
-
Ardenkjær-Larsen ...
-
Palm, Fredrik, 1 ...
-
Stødkilde-Jørgen ...
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinska och f ...
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinska och f ...
-
och Fysiologi
- Artiklar i publikationen
-
American Journal ...
- Av lärosätet
-
Uppsala universitet
-
Linköpings universitet