Expression of Transient Receptor Potential Channels in the Purified Human Pancreatic beta-Cells

• Objective: Members of the transient receptor potential (TRP) channels are involved in mediating the electrical excitability and stimulus-secretion coupling in the pancreatic beta-cells. The expression and the relative abundance of different TRP channels in the human beta-cells are unknown. The objective of this study was to examine the expression of the TRP channels and their relative abundance in the human beta-cell. Methods: RNA sequencing data obtained from human islets, fluorescence-activated cell sorting-purified human beta-cell and human pancreatic acinar cells were analyzed. Gene counts and fragments per kilobase per million mapped reads were obtained. Results: Among the TRPC family only the TRPC1 was expressed in the human beta-cell. TRPV1 channels were not expressed in the human beta-cells. Among the TRPM family, TRPM4, TRPM7, TRPM2, and TRPM3 were expressed in the human beta-cell. Of the remaining TRP channels, TRPP2, TRPML1, and TRPML3 were expressed in these cells. Conclusions: By analyzing the RNA sequencing data, we have detected for the first time the TRP channels that are expressed in the purified human beta-cells, in comparison to the other relevant pancreatic cell types. Our study provides an opportunity to focus on these TRP channels for a better understanding of the electrophysiology and stimulus-secretion coupling in these cells.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Gastroenterologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Gastroenterology and Hepatology (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)

Keyword

transient receptor potential channel

pancreatic beta-cells

RNA sequencing

calcium signaling

islets of Langerhans

signal transduction

Publication and Content Type

ref (subject category)

art (subject category)