Sökning: WFRF:(Chen Yongjun)
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Influence of peptid...
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Chen, XiaomeiUniv Michigan, Coll Pharm, Dept Pharmaceut Sci, 428 Church St, Ann Arbor, MI 48109 USA.
(författare)
Influence of peptide transporter 2 (PEPT2) on the distribution of cefadroxil in mouse brain : A microdialysis study
- Artikel/kapitelEngelska2017
Förlag, utgivningsår, omfång ...
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PERGAMON-ELSEVIER SCIENCE LTD,2017
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-321784
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-321784URI
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https://doi.org/10.1016/j.bcp.2017.02.005DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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Peptide transporter 2 (PEPT2) is a high-affinity low-capacity transporter belonging to the proton-coupled oligopeptide transporter family. Although many aspects of PEPT2 structure-function are known, including its localization in choroid plexus and neurons, its regional activity in brain, especially extracellular fluid (ECF), is uncertain. In this study, the pharmacokinetics and regional brain distribution of cefadroxil, a beta-lactam antibiotic and PEN 2 substrate, were investigated in wildtype and Pept2 null mice using in vivo intracerebral microdialysis. Cefadroxil was infused intravenously over 4 h at 0.15 mg/min/kg, and samples obtained from plasma, brain ECF, cerebrospinal fluid (CSF) and brain tissue. A permeability surface area experiment was also performed in which 0.15 mg/min/kg cefadroxil was infused intravenously for 10 min, and samples obtained from plasma and brain tissues. Our results showed that PEPT2 ablation significantly increased the brain ECF and CSF levels of cefadroxil (2- to 2.5-fold). In contrast, there were no significant differences between wildtype and Pept2 null mice in the amount of cefadroxil in brain cells. The unbound volume of distribution of cefadroxil in brain was 60% lower in Pept2 null mice indicating an uptake function for PEPT2 in brain cells. Finally, PEPT2 did not affect the influx clearance of cefadroxil, thereby, ruling out differences between the two genotypes in drug entry across the blood-brain barriers. These findings demonstrate, for the first time, the impact of PEPT2 on brain ECF as well as the known role of PEPT2 in removing peptide-like drugs, such as cefadroxil, from the CSF to blood.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Keep, Richard F.Univ Michigan Hlth Syst, Dept Neurosurg, Ann Arbor, MI USA.
(författare)
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Liang, YanUniv Michigan, Coll Pharm, Dept Clin Pharm, 428 Church St, Ann Arbor, MI 48109 USA.
(författare)
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Zhu, Hao-JieUniv Michigan, Coll Pharm, Dept Clin Pharm, 428 Church St, Ann Arbor, MI 48109 USA.
(författare)
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Hammarlund-Udenaes, MargaretaUppsala universitet,Institutionen för farmaceutisk biovetenskap(Swepub:uu)marghamm
(författare)
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Hu, YongjunUniv Michigan, Coll Pharm, Dept Pharmaceut Sci, 428 Church St, Ann Arbor, MI 48109 USA.
(författare)
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Smith, David E.Univ Michigan, Coll Pharm, Dept Pharmaceut Sci, 428 Church St, Ann Arbor, MI 48109 USA.
(författare)
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Univ Michigan, Coll Pharm, Dept Pharmaceut Sci, 428 Church St, Ann Arbor, MI 48109 USA.Univ Michigan Hlth Syst, Dept Neurosurg, Ann Arbor, MI USA.
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Biochemical Pharmacology: PERGAMON-ELSEVIER SCIENCE LTD131, s. 89-970006-29521356-1839
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