Pick the Right Pocket. Sub-sampling of Bone Sections to Investigate Diagenesis and DNA Preservation

• Many archaeological bones display a heterogeneous degradation pattern. Highly degraded bones could contain pockets of well-preserved bone, harbouring good quality DNA. This dichotomy may explain why the relationships between global bone preservation parameters such as histological integrity, bone mineral crystallinity or collagen yield, and bulk DNA preservation/amplification success rate have been found to be at best, weak to moderate. In this pilot study, we explore whether or not a more localised approach will highlight a stronger relationship between diagenetic parameters and DNA preservation. This study includes a detailed histological characterisation of bone diagenesis in sub-areas of three bone samples. Regions of the same bone, which displayed differential degrees of preservation or type of diagenesis were sampled for further analysis and both genetic (small scale Illumina MiSeq sequencing) and chemical (Fourier-transform infrared spectrometric analysis) analyses were performed. The aim was to investigate how bone diagenetic processes relate to DNA preservation at a higher resolution than in previous studies. This is key in order to improve DNA analytical success rates. The expected relationship between bone and DNA preservation (retrieved endogenous DNA) was observed and the results corroborate previous work that DNA preservation is linked to the integrity of bone collagen and mineral. The results further suggest that non-biological diagenetic alterations such as etching and the presence of mineral infiltrations and inclusions have a negative effect on DNA preservation/extraction. Copyright (C) 2016 John Wiley & Sons, Ltd.

Ämnesord

HUMANIORA  -- Historia och arkeologi -- Arkeologi (hsv//swe)

HUMANITIES  -- History and Archaeology -- Archaeology (hsv//eng)

Nyckelord

bone

DNA

diagenesis

histology

preservation

bioerosion

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)