Search: WFRF:(Varghese Oommen P. 1977 ) >
Saline catalyse oxi...
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Wang, ShujiangUppsala universitet,Polymerkemi
(author)
Saline catalyse oxime reaction at physiological pH : overcoming a major limitation of bioorthogonal reaction
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LIBRIS-ID:oai:DiVA.org:uu-328905
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-328905URI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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We have discovered a simple and versatile reaction condition for oxime mediated bioconjugation reaction that could be adapted for both aldehyde and keto substrates. We found that saline accelerated the oxime kinetics in a concentration dependent manner under physiological conditions. The reaction mechanism is validated by computational studies, and the versatility of the reaction is demonstrated by cell-surface labeling experiments. Saline offers an efficient and non-toxic catalytic option for performing the bioorthogonal-coupling reaction of biomolecules at the physiological pH. This saline mediated bioconjugation reaction represents the most bio-friendly, mild and versatile approach for conjugating sensitive biomolecules and does not require any extensive purification step.
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Nawale, Ganesh N.Uppsala universitet,Polymerkemi(Swepub:uu)ganna194
(author)
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Kadekar, SandeepUppsala universitet,Polymerkemi(Swepub:uu)sanka861
(author)
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Oommen, Oommen P.Tampere University of Technology, and BioMediTech Institute, Finland
(author)
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Jena, Naresh K.Uppsala universitet,Materialteori(Swepub:uu)narje540
(author)
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Chakraborty, SudipUppsala universitet,Materialteori(Swepub:uu)sudch599
(author)
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Hilborn, Jöns,1956-Uppsala universitet,Polymerkemi(Swepub:uu)johil255
(author)
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Varghese, Oommen P.,1977-Uppsala universitet,Polymerkemi(Swepub:uu)oopva021
(author)
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Uppsala universitetPolymerkemi
(creator_code:org_t)
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