Host cell recruitment patterns by bone morphogenetic protein-2 releasing hyaluronic acid hydrogels in a mouse subcutaneous environment

Todeschi, Maria R.Univ Genoa, Dept Expt Med DIMES, Genoa, Italy.;IRCCS Policlin San Martino, Genoa, Italy. (författare)

Aim: This study aimed to identify host cell recruitment patterns in a mouse model in response to rhBMP-2 releasing hyaluronic acid hydrogels and influence of added nano-hydroxyapatite particles on rhBMP-2 release and pattern of bone formation. Materials & methods: Implanted gels were retrieved after implantation and cells were enzymatically dissociated for flow cytometric analysis. Percentages of macrophages, progenitor endothelial cells and putative mesenchymal stem cells were measured. Implants were evaluated for BMP-2 release by ELISA and by histology to monitor tissue formation. Results & conclusion: Hyaluronic acid+BMP-2 gels influenced the inflammatory response in the bone healing microenvironment. Host-derived putative mesenchymal stem cells were major contributors. Addition of hydroxyapatite nanoparticles modified the release pattern of rhBMP-2, resulting in enhanced bone formation.

El Backly, Rania M.Univ Genoa, Dept Expt Med DIMES, Genoa, Italy.;IRCCS Policlin San Martino, Genoa, Italy.;Alexandria Univ, Conservat Dent Dept, Endodont, Fac Dent, Alexandria, Egypt. (författare)
Varghese, Oommen P.,1977-Uppsala universitet,Polymerkemi(Swepub:uu)oopva021 (författare)
Hilborn, Jöns,1956-Uppsala universitet,Polymerkemi(Swepub:uu)johil255 (författare)
Cancedda, RanieriUniv Genoa, Dept Expt Med DIMES, Genoa, Italy.;IRCCS Policlin San Martino, Genoa, Italy. (författare)
Mastrogiacomo, MaddalenaUniv Genoa, Dept Expt Med DIMES, Genoa, Italy.;IRCCS Policlin San Martino, Genoa, Italy. (författare)
Univ Genoa, Dept Expt Med DIMES, Genoa, Italy.;IRCCS Policlin San Martino, Genoa, Italy.Univ Genoa, Dept Expt Med DIMES, Genoa, Italy.;IRCCS Policlin San Martino, Genoa, Italy.;Alexandria Univ, Conservat Dent Dept, Endodont, Fac Dent, Alexandria, Egypt. (creator_code:org_t)

Ingår i:Regenerative Medicine: FUTURE MEDICINE LTD12:5, s. 525-5391746-07511746-076X

Av författaren/redakt...: Todeschi, Maria ...; El Backly, Rania ...; Varghese, Oommen ...; Hilborn, Jöns, 1 ...; Cancedda, Ranier ...; Mastrogiacomo, M ...

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